Proteomic characterization of donkey milk 'caseome'
| Popis souboru: | ELETTRONICO |
|---|---|
| Jazyk: | English |
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be54a7b5c1e56d672107ad5cd18f5c61 http://hdl.handle.net/11588/371424 |
| Rights: | CLOSED |
| Přírůstkové číslo: | edsair.doi.dedup.....be54a7b5c1e56d672107ad5cd18f5c61 |
| Autor: | Pasquale Ferranti, Carmela De Simone, Maria Grazia Calabrese, Maria Quarto, Giuseppina Garro, Lina Chianese, Francesco Addeo, Luigi Ramunno, Gianfranco Cosenza, Rosalba Mauriello |
| Přispěvatelé: | Chianese, Lina, Calabrese, M. G., Ferranti, Pasquale, Mauriello, Rosalba, Garro, Giuseppina, DE SIMONE, C., Quarto, Maria, Addeo, Francesco, Cosenza, Gianfranco, Ramunno, Luigi |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Proteomics
Molecular Sequence Data Alpha (ethology) Biochemistry donkey Analytical Chemistry chemistry.chemical_compound Complementary DNA Animals Electrophoresis Gel Two-Dimensional Amino Acid Sequence Peptide sequence Gel electrophoresis milk Chromatography biology Chemistry Coomassie Brilliant Blue Organic Chemistry Protein primary structure Caseins Proteomic General Medicine Equidae Molecular Weight Polyclonal antibodies biology.protein Cattle Donkey |
| Popis: | At present, compared with bovine milk, the characterization of donkey milk caseins is at a relatively early stage progress, and only limited data are related to its genetic polymorphism. In this work, the heterogeneity of donkey caseome was investigated using a proteomic approach, based on one- (PAGE, UTLIEF) and two-dimensional (PAGE-->UTLIEF) electrophoresis, stained with either Coomassie Brilliant Blue or specific polyclonal antibodies, and structural MS analysis. These combined methodologies allowed the contemporary identification of donkey alpha(s1), alpha(s2), beta and kappa-CN with their related heterogeneity due to phosphorylation (alpha(s1), alpha(s2) and beta-CN), glycosylation (kappa-CN) and incorrect splicing of RNA in mRNA (deleted forms of alpha(s1)-CN and beta-CN). The results achieved showed 11 components for kappa-CN, six phosphorylated components for beta and alpha(s1)-CN and three main phosphorylated components for alpha(s2)-CN, each accounting for 10, 11 and 12 P/mole. At this regard, for the first time, the primary structure of the expressed protein corresponding to the only available donkey alpha(s2)-CN cDNA sequence was determined. Furthermore beta-CN was found in homozygous and heterozygous state for the occurrence of a genetic beta-CN variant having a MW value 28 mass units higher than the common beta-CN phenotype. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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