Extended-spectrum beta-lactamases and plasmid diversity in urinary isolates of Escherichia coli in Croatia: a nation-wide, multicentric, retrospective study
| Autor: | Maja Tomić-Paradžik, Ivan Barišić, Vesna Elveđi-Gašparović, Zoran Herljević, Nataša Beader, Rick Conzemius, Branka Bedenić, Jasmina Vraneš, Marija Krilanović, Tomislav Meštrović |
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| Rok vydání: | 2020 |
| Předmět: |
clone (Java method)
CTX-M beta-lactamase type E. coli ESBL Plasmid Urinary tract infection Genotype Croatia medicine.drug_class Cephalosporin Microbial Sensitivity Tests Biology Serogroup medicine.disease_cause Microbiology beta-Lactamases 03 medical and health sciences Drug Resistance Bacterial Escherichia coli Prevalence polycyclic compounds medicine Humans Gene Genotyping Escherichia coli Infections Retrospective Studies 030304 developmental biology Whole genome sequencing Molecular Epidemiology 0303 health sciences 030306 microbiology Genetic Variation General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses Antimicrobial Anti-Bacterial Agents 3. Good health Urinary Tract Infections Genome Bacterial Plasmids |
| Zdroj: | Folia Microbiologica |
| ISSN: | 0015-5632 |
| DOI: | 10.1007/s12223-019-00769-1 |
| Popis: | In recent years, a dramatic increase in the prevalence of Escherichia coli strains producing extended-spectrum β-lactamases (ESBLs) has been observed - both in the community and in healthcare settings. This multicentric study aimed to characterize ESBLs produced by E. coli isolates causing hospital- onset and community urinary tract infections, as well as to compare their antimicrobial sensitivity patterns, β-lactamase content and plasmid types. Phenotypic tests for the detection of ESBLs and plasmid-mediated AmpC β- lactamases were initially pursued, followed by molecular detection of resistance genes, plasmid characterization, genotyping with pulsed-field gel electrophoresis and whole genome sequencing (WGS). The isolates exhibited high level of resistance to expanded-spectrum cephalosporins (ESC) and carried CTX-M (cefotaximase-Munich) or TEM (Temoniera) β- lactamases. All six representative isolates subjected to WGS belonged to the widespread clone ST131. In conclusion, our study demonstrated dissemination of group 1 CTX-M positive E. coli in different geographic regions of Croatia, but also different components of the health care systems (hospitals, nursing homes and the community) and confirmed the switch from SHV-2 (suphydril variant) and SHV-5 ESBLs to the nation-wide predominance of group 1 CTX-M β-lactamases. Different plasmids were shown to be associated with the dissemination of blaCTX-M genes in different geographic regions of Croatia. |
| Databáze: | OpenAIRE |
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