Elastin-derived scaffolding associated or not with bone morphogenetic protein (BMP) or hydroxyapatite (HA) in the repair process of metaphyseal bone defects

Autor: Arnaldo Rodrigues Santos Junior, Fernando Bento Cunha, Guinea Brasil Camargo Cardoso, Karina Torres Pomini, Daniela Vieira Buchaim, Marco Antonio Hungaro Duarte, M. P. Alcalde, Rogério Leone Buchaim, Eduardo Gomes Machado, Renato de Moraes, Ana Maria de Guzzi Plepis, Marcelo Rodrigues da Cunha, Virginia da Conceição Amaro Martins, M.A.S. Munhoz
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
Pathology
Bone Regeneration
Time Factors
02 engineering and technology
Matrix (biology)
Biochemistry
Diagnostic Radiology
Extracellular matrix
Osteogenesis
ELASTINA
Medicine and Health Sciences
Femur
Von Kossa stain
Musculoskeletal System
Materials
Extracellular Matrix Proteins
0303 health sciences
Multidisciplinary
Tissue Scaffolds
biology
Chemistry
Radiology and Imaging
021001 nanoscience & nanotechnology
Bone Imaging
Physical Sciences
Bone Morphogenetic Proteins
Chronic inflammatory response
Engineering and Technology
Medicine
Anatomy
0210 nano-technology
Research Article
Biotechnology
medicine.medical_specialty
Histology
Imaging Techniques
Science
Materials Science
Surgical and Invasive Medical Procedures
Bioengineering
Bone healing
Research and Analysis Methods
Bone morphogenetic protein
Biomaterials
03 medical and health sciences
Diagnostic Medicine
medicine
Animals
Humans
Skeleton
030304 developmental biology
Tissue Engineering
Biology and Life Sciences
Proteins
X-Ray Microtomography
Elastin
Rats
Disease Models
Animal
Durapatite
biology.protein
Collagens
Zdroj: PLoS ONE, Vol 15, Iss 4, p e0231112 (2020)
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
PLoS ONE
ISSN: 1932-6203
Popis: Tissue engineering represents a promising alternative for reconstructive surgical procedures especially for the repair of bone defects that do not regenerate spontaneously. The present study aimed to evaluate the effects of the elastin matrix (E24/50 and E96/37) incorporated with hydroxyapatite (HA) or morphogenetic protein (BMP) on the bone repair process in the distal metaphysis of rat femur. The groups were: control group (CG), hydrolyzed elastin matrix at 50°C/24h (E24/50), E24/50 + HA (E24/50/HA), E24/50 + BMP (E24/50/BMP), hydrolyzed elastin matrix at 37°C/96h (E96/37), E96/37 + HA (E96/37/HA), E96/37 + BMP (E96/37/BMP). Macroscopic and radiographic analyses showed longitudinal integrity of the femur in all groups without fractures or bone deformities. Microtomographically, all groups demonstrated partial closure by mineralized tissue except for the E96/37/HA group with hyperdense thin bridge formation interconnecting the edges of the ruptured cortical. Histologically, there was no complete cortical recovery in any group, but partial closure with trabecular bone. In defects filled with biomaterials, no chronic inflammatory response or foreign body type was observed. The mean volume of new bone formed was statistically significant higher in the E96/37/HA and E24/50 groups (71.28 ± 4.26 and 66.40 ± 3.69, respectively) than all the others. In the confocal analysis, it was observed that all groups presented new bone markings formed during the experimental period, being less evident in the CG group. Von Kossa staining revealed intense calcium deposits distributed in all groups. Qualitative analysis of collagen fibers under polarized light showed a predominance of red-orange birefringence in the newly regenerated bone with no difference between groups. It was concluded that the E24/50 and E96/37/HA groups promoted, with greater speed, the bone repair process in the distal metaphysis of rat femur.
Databáze: OpenAIRE
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