Sequence analysis and spatiotemporal developmental distribution of the Cat-1-type transporter slc7a1a in zebrafish (Danio rerio)
| Autor: | Anders Fjose, Tiziano Verri, Ståle Ellingsen, Shailesh Narawane, Ivar Rønnestad |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Embryo
Nonmammalian animal structures Physiology Sequence analysis Danio Spatiotemporal expression In situ hybridization slc7a1b Aquatic Science slc7a1a Eye Biochemistry Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Sequence Analysis Protein Complementary DNA Animals Amino Acid Sequence Gene Zebrafish Phylogeny Cationic Amino Acid Transporter 1 030304 developmental biology chemistry.chemical_classification 0303 health sciences Base Sequence biology fungi Brain Gene Expression Regulation Developmental Nephrons Sequence Analysis DNA General Medicine Zebrafish Proteins Ornithine biology.organism_classification Amino acid Cell biology Branchial Region Somites chemistry embryonic structures Cationic amino acid transport(ers) 030217 neurology & neurosurgery |
| Zdroj: | Fish Physiology & Biochemistry Fish Physiology and Biochemistry 2281–2298 |
| Popis: | Cationic amino acid transporter 1 (Cat-1 alias Slc7a1) is a Na+-independent carrier system involved in transport and absorption of the cationic amino acids lysine, arginine, histidine, and ornithine and has also been shown to be indispensable in a large variety of biological processes. Starting from isolated full-length zebrafish (Danio rerio) cDNA for slc7a1a, we performed comparative and phylogenetic sequence analysis, investigated the conservation of the gene during vertebrate evolution, and defined tissue expression during zebrafish development. Whole mount in situ hybridization first detected slc7a1a transcripts in somites, eyes, and brain at 14 h post-fertilization (hpf) with additional expression in the distal nephron at 24 hpf and in branchial arches at 3 days post-fertilization (dpf), with significant increase by 5 dpf. Taken together, the expression analysis of the zebrafish Cat-1 system gene slc7a1a suggests a functional role(s) during the early development of the central nervous system, muscle, gills, and kidney. |
| Databáze: | OpenAIRE |
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