Benzalkonium chloride-induced myofibroblastic transdifferentiation of Tenon’s capsule fibroblasts is inhibited by coculture with corneal epithelial cells or by interleukin-10
| Autor: | Tadahiko Ogata, Manami Ota, Kazuhiro Tokuda, Shinichiro Teranishi, Sho-Hei Uchi, Takuya Yoshimoto, Masaaki Kobayashi, Chiemi Yamashiro, Makoto Hatano, Makiko Wakuta, Atsushige Ashimori, Fumiaki Higashijima, Kazuhiro Kimura, Yuka Kobayashi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Tenon Capsule
Science Immunofluorescence Article Cornea Benzalkonium chloride Tenon's capsule Fibrosis medicine Humans Myofibroblasts Eye diseases Cells Cultured Corneal epithelium Cell Proliferation Multidisciplinary Molecular medicine medicine.diagnostic_test Chemistry Transdifferentiation Epithelial Cells Fibroblasts medicine.disease Molecular biology Actins Coculture Techniques Interleukin-10 Interleukin 10 medicine.anatomical_structure Cell Transdifferentiation Trans-Activators Medicine sense organs Benzalkonium Compounds Myofibroblast medicine.drug Signal Transduction |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Benzalkonium chloride (BAC) is used as a preservative in eyedrops but induces subconjunctival fibrosis that can result in failure of glaucoma surgery. Tenon’s capsule fibroblasts in subconjunctival tissue interact with the corneal epithelium through tear fluid. With the use of a coculture system, we have now investigated the effect of human corneal epithelial (HCE) cells on myofibroblastic transdifferentiation of human Tenon fibroblasts (HTFs) induced by BAC (5 × 10−6%). Immunofluorescence and immunoblot analyses revealed that the BAC-induced expression of α smooth muscle actin (αSMA) in HTFs was suppressed by coculture of these cells with HCE cells (p p p p |
| Databáze: | OpenAIRE |
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