A decadentate Gd(III)-coordinating paramagnetic cosolvent for protein relaxation enhancement measurement
| Autor: | Zhou Gong, Xin-Hua Gu, Wei-Ping Zhang, Chun Tang, Da-Chuan Guo |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Magnetic Resonance Spectroscopy
Chemistry Gadolinium Relaxation (NMR) Analytical chemistry Electron Spin Resonance Spectroscopy chemistry.chemical_element Proteins Water Nuclear magnetic resonance spectroscopy Biochemistry Solvent Paramagnetism Protein structure Yield (chemistry) Physical chemistry Molecule Nuclear Magnetic Resonance Biomolecular Spectroscopy Edetic Acid |
| Zdroj: | Journal of biomolecular NMR. 58(3) |
| ISSN: | 1573-5001 |
| Popis: | Solvent paramagnetic relaxation enhancement (sPRE) arises from random collisions between paramagnetic cosolvent and protein of interest. The sPRE can be readily measured, affording protein structure information. However, lack of an inert cosolvent probe may yield sPRE values that are not consistent with protein structure. Here we synthesized a new sPRE probe, triethylenetetraamine hexaacetate trimethylamide gadolinium, or Gd(III)–TTHA–TMA. With a total of 10 coordination sites, this paramagnetic cosovlent eliminates an inner-sphere water molecule that can otherwise transfer relaxation to protein through exchange. With the metal ion centered, the new probe is largely spherical with a radius of 4.0 A, permitting accurate back calculation of sPRE. The effectiveness Gd(III)–TTHA–TMA as a sPRE probe was demonstrated on three well-studied protein systems. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink