ClZISO mutation leads to photosensitive flesh in watermelon

Autor: Jie Zhang, Honghe Sun, Shaogui Guo, Yi Ren, Maoying Li, Jinfang Wang, Yongtao Yu, Haiying Zhang, Guoyi Gong, Hongju He, Chao Zhang, Yong Xu
Rok vydání: 2022
Předmět:
Zdroj: Theoretical and Applied Genetics. 135:1565-1578
ISSN: 1432-2242
0040-5752
DOI: 10.1007/s00122-022-04054-7
Popis: The mutation of ClZISO identified in EMS-induced watermelon leads to photosensitive flesh in watermelon. Watermelon (Citrullus lanatus) has a colorful flesh that attracts consumers and benefits human health. We developed an ethyl-methanesulfonate mutation library in red-fleshed line '302' to create new flesh color lines and found a yellow-fleshed mutant which accumulated ζ-carotene. The initial yellow color of this mutant can be photobleached within 10 min under intense sunlight. A long-term light-emitting diode (LED) light treatment turned flesh color from yellow to pink. We identified this unique variation as photosensitive flesh mutant ('psf'). Using bulked segregant analysis, we fine-mapped an EMS-induced G-A transversion in 'psf' which leads to a premature stop codon in 15-cis-ζ-carotene isomerase (ClZISO) gene. We detected that wild-type ClZISO is expressed in chromoplasts to catalyze the conversion of 9,15,9'-tri-cis-ζ-carotene to 9,9'-di-cis-ζ-carotene. The truncated ClZISO
Databáze: OpenAIRE
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