Liver fibrosis during the development of biliary atresia: Proof of principle in the murine model
| Autor: | F.H.M. Morsink, Claudia M. G. Keyzer-Dekker, G. J. A. Offerhaus, Henkjan J. Verkade, F.J.W. ten Kate, J. F. Kuebler, Jan B F Hulscher, Claus Petersen, Robert C. Lind |
|---|---|
| Přispěvatelé: | Center for Liver, Digestive and Metabolic Diseases (CLDM), Lifestyle Medicine (LM), Pediatric Surgery |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Liver Cirrhosis
EXPRESSION medicine.medical_specialty Pathology Research Support medicine.disease_cause MOUSE Severity of Illness Index Pediatrics Gastroenterology Rotavirus Infections Key words Biliary atresia Pathogenesis Fibrosis Biliary atresia Internal medicine Rotavirus medicine Journal Article INJURY Animals Pediatrics Perinatology and Child Health Non-U.S. Gov't Rhesus rotavirus murine model Mice Inbred BALB C BILE-DUCT LIGATION MESENCHYMAL TRANSITION Bile duct business.industry Research Support Non-U.S. Gov't General Medicine medicine.disease Perinatology Immunohistochemistry Bile duct proliferation Staining and Child Health Disease Models Animal MICE medicine.anatomical_structure Liver Pediatrics Perinatology and Child Health Disease Progression Surgery OBSTRUCTION business |
| Zdroj: | Journal of Pediatric Surgery, 50(8), 1304-1309. W B SAUNDERS CO-ELSEVIER INC Journal of Pediatric Surgery, 50(8), 1304. W.B. Saunders Ltd Journal of Pediatric Surgery, 50(8), 1304-1309. W.B. Saunders |
| ISSN: | 0022-3468 |
| Popis: | Background: The murine model of biliary atresia (BA) is used for examining the pathogenesis of BA. The aim of the study was description of the morphological features and illustrating the detailed development of fibrosis using the Biliary Atresia Research Consortium (BARC) system.Methods: Neonatal mice were injected intraperitoneally with rhesus rotavirus (RRV) strain (N = 17). Healthy mice were the control group (N = 29). All mice were sacrificed at 7 or 14 days after birth. Two pathologists examined the morphological features using the BARC system; CK19, alpha SMA and collagen type I were assessed by immunohistochemistry.Results: In RRV mice, portal fibrous expansion with focal bile duct proliferation and strong portal cellular infiltrate was found in contrast to healthy mice. In RRV mice, CK19 bile duct staining was significantly less or absent (p Conclusions: The morphological features observed in the murine model of BA correspond with the BA characteristics according to the BARC criteria. Fibrosis is an important feature of the model. Therefore, this murine model is useful for investigating the pathogenesis of BA. (C) 2015 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect