Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae
| Autor: | V. Kava Cordeiro, E.A. Luna Alves Lima, João Lúcio de Azevedo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
biology
Mutant fungi auxotrophk mutants gamma radiation Wild type ultraviolet light Metarhizium anisopliae biology.organism_classification nitrous acid mutagenic agents Conidium Microbiology Entomopathogenic fungus Ultraviolet light Animal Science and Zoology Aminoacid biosynthesis Proline Agronomy and Crop Science colour mutants |
| Zdroj: | Scientia Agricola v.52 n.3 1995 Scientia Agrícola Universidade de São Paulo (USP) instacron:USP |
| Popis: | A wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%) and nitrous acid (0.06%).The conidial colour and auxotrophk mutants isolated until now from M. anisopliae were reviewed. |
| Databáze: | OpenAIRE |
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