In-Depth Analysis of Egg-Tempera Paint Layers by Multiphoton Excitation Fluorescence Microscopy

Autor: Mikel Sanz, Sotiris Psilodimitrakopoulos, Raffaella Fontana, Marcin Sylwestrzak, Riccardo Cicchi, Andreas Lemonis, George Filippidis, Piotr Targowski, Mohamed Oujja, Sara Mattana, Marta Castillejo, Alice Dal Fovo, Chiara Grazia, Aldo Romani
Přispěvatelé: European Commission, Ministerio de Economía y Competitividad (España), Comunidad de Madrid
Rok vydání: 2020
Předmět:
Materials science
Photoluminescence
Optical fiber
Opacity
Infrared
Geography
Planning and Development
TJ807-830
02 engineering and technology
Management
Monitoring
Policy and Law
TD194-195
01 natural sciences
Renewable energy sources
law.invention
Optics
Optical coherence tomography
nonlinear optical microscopy
multi-photon excitation fluorescence
optical coherence tomography
egg-tempera paintings
non-invasive stratigraphy
law
11. Sustainability
medicine
GE1-350
Laser-induced fluorescence
medicine.diagnostic_test
Environmental effects of industries and plants
Renewable Energy
Sustainability and the Environment
business.industry
010401 analytical chemistry
Non-invasive stratigraphy
Nonlinear optical microscopy
021001 nanoscience & nanotechnology
Laser
Multi-photon excitation fluorescence
0104 chemical sciences
3. Good health
Environmental sciences
Femtosecond
Egg-tempera paintings
0210 nano-technology
business
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
Sustainability, Vol 12, Iss 3831, p 3831 (2020)
Sustainability
Volume 12
Issue 9
Popis: © 2020 by the authors.
The non-invasive depth-resolved imaging of pictorial layers in paintings by means of linear optical techniques represents a challenge in the field of Cultural Heritage (CH). The presence of opaque and/or highly-scattering materials may obstruct the penetration of the radiation probe, thus impeding the visualization of the stratigraphy of paintings. Nonlinear Optical Microscopy (NLOM), which makes use of tightly-focused femtosecond pulsed lasers as illumination sources, is an emerging technique for the analysis of painted objects enabling micrometric three-dimensional (3D) resolution with good penetration capability in semi-transparent materials. In this work, we evaluated the potential of NLOM, specifically in the modality of Multi-Photon Excitation Fluorescence (MPEF), to probe the stratigraphy of egg-tempera mock-up paintings. A multi-analytical non-invasive approach, involving ultraviolet-visible-near infrared (UV-Vis-NIR) Fiber Optics Reflectance Spectroscopy, Vis-NIR photoluminescence, and Laser Induced Fluorescence, yielded key-information for the characterization of the constituting materials and for the interpretation of the nonlinear results. Furthermore, the use of three nonlinear optical systems allowed evaluation of the response of the analyzed paints to different excitation wavelengths and photon doses, which proved useful for the definition of the most suitable measurement conditions. The micrometric thickness of the paint layers, which was not measurable by means of Optical Coherence Tomography (OCT), was instead assessed by MPEF, thus demonstrating the effectiveness of this nonlinear modality in probing highly-scattering media, while ensuring the minimal photochemical disturbance to the examined materials.
This research was funded by the European H2020 IPERION CH Project (Integrated Platform for the European Research Infrastructure ON Cultural Heritage (GA 654028, WP6); Spanish State Research Agency (AEI) and European Regional Development (FEDER) through Project CTQ2016-75880-P-AEI/FEDER, UE; and TOP Heritage-CM (S2018/NMT-4372) from the Community of Madrid and with the support of Plataforma Temática Interdisciplinar of CSIC “Patrimonio Abierto: Investigación y Sociedad” (PTI-PAIS).
Databáze: OpenAIRE
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