The G-Protein-Coupled Membrane Estrogen Receptor Is Present in Horse Cryptorchid Testes and Mediates Downstream Pathways

Autor: Piotr Pawlicki, Malgorzata Kotula-Balak, Magdalena Profaska-Szymik, Michal Duliban, Anna Galuszka, Bartosz J. Płachno, Maciej Witkowski, Laura Pardyak, Samuel Kantor
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Male
Membrane estrogen receptor
Receptors
G-Protein-Coupled
0302 clinical medicine
Testis
Biology (General)
Spectroscopy
medicine.diagnostic_test
Chemistry
General Medicine
Immunohistochemistry
Computer Science Applications
horse
Seminiferous tubule
medicine.anatomical_structure
Receptors
Estrogen
GPER
cryptorchidism
Intracellular
Signal Transduction
estrogens
medicine.medical_specialty
medicine.drug_class
QH301-705.5
Blotting
Western
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Western blot
GTP-Binding Proteins
Internal medicine
medicine
Animals
Horses
G protein-coupled receptor
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
Organic Chemistry
030104 developmental biology
Endocrinology
Estrogen
Microscopy
Electron
Scanning
Horse Diseases
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 7131, p 7131 (2021)
Volume 22
Issue 13
ISSN: 1422-0067
Popis: Cryptorchidism in horses is a commonly occurring malformation. The molecular basis of this pathology is not fully known. In addition, the origins of high intratesticular estrogen levels in horses remain obscure. In order to investigate the role of the G-protein-coupled membrane estrogen receptor (GPER) and establish histological and biochemical cryptorchid testis status, healthy and cryptorchid horse testes were subjected to scanning electron microscopy analysis, histochemical staining for total protein (with naphthol blue black
NBB), acid content (with toluidine blue O
TBO), and polysaccharide content (with periodic acid–Schiff
PAS). The expression of GPER was analyzed by immunohistochemistry and Western blot. GPER-mediated intracellular cAMP and calcium (Ca2+) signaling were measured immunoenzymatically or colorimetrically. Our data revealed changes in the distribution of polysaccharide content but not the protein and acid content in the cryptorchid testis. Polysaccharides seemed to be partially translocated from the interstitial compartment to the seminiferous tubule compartment. Moreover, the markedly decreased expression of GPER and GPER downstream molecules, cAMP and Ca2+, suggests their potential role in testis pathology. Increased estrogen levels in cryptorchid conditions may be linked to disturbed GPER signaling. We postulate that GPER is a prominent key player in testis development and function and may be used as a new biomarker of horse testis in health and disease.
Databáze: OpenAIRE
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