Antibodies toTrichomonas vaginalissurface glycolipid
| Autor: | Lynette B. Corbeil, Paul D. Stamper, Gregory C. Gray, R R Corbeil, F D Bastida-Corcuera, Bibhuti N. Singh, M Davuluri, Karen M. Schlangen |
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| Rok vydání: | 2013 |
| Předmět: |
Sexually transmitted disease
medicine.drug_class Antibodies Protozoan Trichomonas Infections Antigens Protozoan Enzyme-Linked Immunosorbent Assay Dermatology medicine.disease_cause Monoclonal antibody Article Epitope Mice Blood serum Antigen Pregnancy Trichomonas vaginalis medicine Animals Humans Prospective Studies Mice Inbred BALB C Trichomoniasis biology business.industry Antibodies Monoclonal medicine.disease Virology Infectious Diseases Immunology biology.protein Female Glycolipids Antibody business |
| Zdroj: | Sexually Transmitted Infections. 89:467-472 |
| ISSN: | 1472-3263 1368-4973 |
| DOI: | 10.1136/sextrans-2012-051013 |
| Popis: | Background Human trichomoniasis is the most common non-viral sexually transmitted disease, yet immune responses are not well studied. Methods Since the Trichomonas vaginalis lipophosphoglycan (TvLPG) is an important virulence factor, a bank of eight monoclonal antibodies was generated to define the antigen in clinical isolates. The TvLPG-specific antibody response of women who were culture positive (n=33) or negative (n=33) for T vaginalis infection was determined by isotype-specific ELISA. Results The bank of monoclonal antibodies reacted with conserved surface TvLPG epitopes in 27 isolates from pregnant women at their first prenatal visit. Conserved TvLPG epitopes were shown to be surface exposed by immunofluorescence. Sera collected from the same patients at the same time were assayed for specific antibodies. Serum and vaginal secretions from 33 T vaginalis-positive women had statistically higher IgG anti-TvLPG levels than age-matched and race-matched negative controls in the same clinical study (p |
| Databáze: | OpenAIRE |
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