Further development of reconstructive and cell tissue-engineering technology for treatment of complete peripheral nerve injury in rats
| Autor: | Zvi Nevo, Abraham Shahar, Lubov Barsky, Moshe Graif, Tami Hayon, Shimon Rochkind, Malvina Alon, Dalia El-Ani, Inbal Odvak, Liliana Astachov |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Reconstructive surgery medicine.medical_specialty Nerve guidance conduit Biocompatible Materials Implants Experimental Tissue engineering Peripheral Nerve Injuries medicine Animals Peripheral Nerves Rats Wistar Tissue Engineering business.industry General Medicine Anatomy Plastic Surgery Procedures Rats Peripheral Surgery Transplantation Electrophysiology Neurology Peripheral nerve injury Neurology (clinical) Epineurial repair business |
| Zdroj: | Neurological Research. 26:161-166 |
| ISSN: | 1743-1328 0161-6412 |
| Popis: | In this work we evaluated the efficacy of biodegradable composite co-polymer guiding neurotube, based on tissue-engineering technology, for the treatment of complete peripheral nerve injury where the nerve defect is significant. The right sciatic nerve of 12 three-month-old rats was completely transected and peripheral nerve segment was removed. A 2.2-cm biodegradable co-polymer neurotube containing viscous gel (NVR-N-Gel) with survival factors, neuroprotective agents and Schwann cells was placed between the proximal and the distal parts of the transected nerve for reconnection a 2-cm nerve defect. The proximal and distal parts of the nerve were fixed into the neurotube using 10-0 sutures. Ultrasound observation showed growth of the axons into the composite neurotube 2 months after the surgery. Electrophysiological study indicated compound muscle action potentials in nine out of 12 rats, 2-4 months after peripheral nerve reconstructive surgery. The postoperative follow-up (up to 4 months) on the operated rats that underwent peripheral nerve reconstruction using composite co-polymer neurotube, showed beginning of re-establishment of active foot movements. The tube was dissolved and nerve showed complete reconnection. Histological observation of the nerve showed growth of myelinated axons into the site where a 2-cm nerve defect replaced by composite co-polymer neurotube and into the distal part of the nerve.(1) an innovative composite neurotube for reconstruction of significant loss of peripheral nerve segment is described; (2) a viscous gel, containing survival factors, neuroprotective agents and Schwann cells served as a regenerative environment for repair. Further investigations of this reconstructive procedure are being conducted. |
| Databáze: | OpenAIRE |
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