Cloning of Genes of the Aminopeptidase T Family fromThermus thermophilusHB8 andBacillus stearothermophilusNCIB8924: Apparent Similarity to the Leucyl Aminopeptidase Family
| Autor: | Shuichi Kaminogawa, Hidemasa Motoshima, Etsuo Minagawa, Fuji Tsukasaki |
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| Rok vydání: | 1997 |
| Předmět: |
Molecular Sequence Data
Sequence alignment Aminopeptidases Applied Microbiology and Biotechnology Biochemistry Aminopeptidase Gene Expression Regulation Enzymologic Substrate Specificity Analytical Chemistry Geobacillus stearothermophilus Leucyl Aminopeptidase Bacterial Proteins Escherichia coli Fluorometry Amino Acid Sequence Cloning Molecular Molecular Biology Peptide sequence Leucyl aminopeptidase Genetics Base Sequence Sequence Homology Amino Acid biology Thermus aquaticus Thermus thermophilus Thermus Organic Chemistry Nucleic acid sequence General Medicine biology.organism_classification Culture Media Molecular Weight Plasmids Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 61:1710-1717 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.61.1710 |
| Popis: | To obtain genes with sequence similarity to aminopeptidase T (AP-T) of Thermus aquaticus YT-1, we cloned the genes encoding aminopeptidase Th (AP-Th) from Thermus thermophilus HB8 and aminopeptidase II (APII) from Bacillus stearothermophilus NCIB8924. The AP-Th gene encoded a polypeptide of 408 amino acid residues and the deduced molecular weight of this subunit was 45,015. The APII gene encoded a polypeptide of 413 amino acid residues with a deduced molecular weight of 46,207. The extent of amino acid sequence similarity between AP-Th and AP-T was 86%, and that between APII and AP-T was 43%. The substrate specificities of these expressed enzymes were similar, and each efficiently hydrolyzed leucyl- or phenyl-peptide substrates. Since the deduced amino acid sequence of these enzymes show no similarity to other known aminopeptidases, they appear to comprise an independent family of peptidases, designated the AP-T family. However, a conserved region within the enzymes of the AP-T family shows similarity to the active site signature of the leucyl aminopeptidase family, suggesting that these enzymes may belong to the leucyl aminopeptidase superfamily. |
| Databáze: | OpenAIRE |
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