An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to maedi-visna virus
| Autor: | Jan Schaake, Dirk J. Houwers, Arno L.J. Gielkens |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
chemistry.chemical_classification
Sheep General Veterinary Visna-maedi virus Complement Fixation Tests Agar gel precipitation Enzyme-Linked Immunosorbent Assay General Medicine Antigen-Antibody Complex Biology Complement fixation test Antibodies Viral Microbiology Virology Horseradish peroxidase Virus Maedi/Visna virus Enzyme chemistry biology.protein Animals Flock Antibody |
| Zdroj: | Veterinary microbiology. 7(3) |
| ISSN: | 0378-1135 |
| Popis: | An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to maedi-visna virus (mvv) in sheep is described, in which microtitre plates are with a partly purified preparation of mvv. The antibodies bound are detected by a horseradish peroxidase conjugate. The results obtained with ELISA on a total of 493 serum samples from several commercial flocks were compared to those of a routine agar gel precipitation test (AGPT) and a complement fixation test (CFT). All samples which scored positive in AGPT, CFT or both (20.8%) were also found positive by ELISA. In addition, with ELISA a further 11.5% of the samples were positive. Serum samples from maedi-free flocks, from sheep suffering from sheep pulmonary adenomatosis and from lambs immunized against other viruses were all negative by ELISA. The assay has been used routinely for some years and proved to be specific, sensitive and suited for screening of large numbers of serum samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|