In vitro intestinal epithelium responses to titanium dioxide nanoparticles
| Autor: | Paola Pedata, Antonella Venezia, Giuseppe Iacomino, Marcella Cammarota, Maria Grazia Volpe, Marco Romano, Giulia Ricci, Vincenzo Guida, Livia Malorni, Chiara Schiraldi, Nunzia Iannaccone |
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| Přispěvatelé: | Pedata, Paola, Ricci, Giulia, Malorni, Livia, Venezia, Antonella, Cammarota, Marcella, Volpe, Maria Grazia, Iannaccone, Nunzia, Guida, Vincenzo, Schiraldi, Chiara, Romano, Marco, Iacomino, Giuseppe |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
030309 nutrition & dietetics
Intestinal permeability 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Intestinal mucosa medicine Humans RNA Messenger Intestinal Mucosa Particle Size Nanomaterials Titanium 0303 health sciences Food additive Toxicity Chemistry Tumor Necrosis Factor-alpha Interleukin-8 04 agricultural and veterinary sciences Food additives medicine.disease Endoplasmic Reticulum Stress Nanomaterial P25 TiO2 040401 food science Intestinal epithelium In vitro Cell culture Titanium dioxide Biophysics Ultrastructure Cytokines Nanoparticles Caco-2 Cells Food Science |
| Zdroj: | Food research international 119 (2018): 634–642. doi:10.1016/j.foodres.2018.10.041 info:cnr-pdr/source/autori:Paola Pedata1, Giulia Ricci1, Livia Malorni2, Antonella Venezia2, Marcella Cammarota1, Maria Grazia Volpe2, Nunzia Iannaccone2, Vincenzo Guida1, Chiara Schirardi1, Marco Romano3, Giuseppe Iacomino2/titolo:In vitro intestinal epithelium responses to titanium dioxide nanoparticles/doi:10.1016%2Fj.foodres.2018.10.041/rivista:Food research international/anno:2018/pagina_da:634/pagina_a:642/intervallo_pagine:634–642/volume:119 |
| DOI: | 10.1016/j.foodres.2018.10.041 |
| Popis: | Titanium dioxide (TiO2) is enclosed in many consumer products including pharmaceuticals, cosmetics, and foods. TiO2 (E171) is daily ingested as mixed nano- and submicron-sized particles since it is approved as a white colorant in Europe in a wide variety of food products, Noteworthy, the relevant risk assessment has never been satisfactorily concluded and growing alarms for human hazards deriving from TiO2 exposure are incrementally reported. The objective of the present study was to establish conceivable mechanisms by which nano-sized TiO2 particles affect physiological function of the intestinal epithelium layer. The well-established Caco-2 cell line differentiated for 21 days on permeable supports was used as a predictive model of the human intestinal mucosa to identify the biological response triggered by TiO2 particles. Exposure to 42 μg/mL TiO2 nanoparticles disrupted the tight junctions-permeability barrier with a prompt effect detectable after 4 h incubation time and wide effects on barrier integrity at 24 h. Transport and ultrastructural localization of TiO2 nanoparticles were determined by ICP-OES, TEM and ESI/EELS analysis, respectively. Nano-sized particles were efficiently internalized and preferentially entrapped by Caco-2 monolayers. Storage of TiO2 nanoparticles inside the cells affected enterocytes viability and triggered the production of pro-inflammatory cytokines, including TNF-α and IL-8. Taken together these data indicate that nano-sized TiO2 particles exert detrimental effects on the intestinal epithelium layer. |
| Databáze: | OpenAIRE |
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