Stability of coagulation parameters in plasma samples at room temperature after one freeze/thaw cycle

Autor: Marion Gaudard, Elodie Boissier, Laurie Talon, Jonathan Douxfils, Anne‐Françoise Sapin, Thomas Sinegre, Aurélien Lebreton
Přispěvatelé: CHU Clermont-Ferrand, Centre hospitalier universitaire de Nantes (CHU Nantes), Université de Namur [Namur] (UNamur), Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA)
Rok vydání: 2021
Předmět:
Zdroj: International Journal of Laboratory Hematology
International Journal of Laboratory Hematology, Wiley, 2022, 44 (3), pp.610-618. ⟨10.1111/ijlh.13794⟩
ISSN: 1751-553X
1751-5521
1365-2257
Popis: INTRODUCTION: Sample freezing is a part of routine laboratory tasks because some coagulation parameters are analysed in batches to optimize reagent consumption. The coagulation parameter stability in fresh and frozen samples has been described, but data are scarcer after thawing. This study objective was to determine the stability of the main coagulation parameters (from blood withdrawn on siliconized CTAD tubes and double-centrifuged) after one freeze/thaw cycle to generate procedures for appropriate handling, storage and testing.METHODS: Prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen, D-dimers, clotting factors (F), protein C, protein S, antithrombin, lupus anticoagulant (LA)-sensitive aPTT and diluted-Russel's viper venom time (dRVVT) were assessed in 60 plasma samples (n=30, normal range and n=30, outside the normal range). Thirty samples from anticoagulated patients [unfractionated heparin (UFH), low-molecular weight heparin (LMWH), apixaban or rivaroxaban] were assessed using specific anticoagulant assays. Frozen samples were thawed, and assays were performed at 15 min, 2, 4 and 6 h after thawing. The coagulation parameter stability was assessed with the method of rejection limit.RESULTS: After thawing, aPTT, PT, fibrinogen, D-dimers, FII, FV, FX, FIX, FXI, FXII, PC and UFH anti-Xa activity remained stable for at least 6 h, FVII for 5 h, PS, AT, dRVVT screen assay and LMWH anti-Xa activity for 4 h, and LA-sensitive aPTT and apixaban-specific anti-Xa activity for 3 h. FVIII, dRVVT confirm assay and rivaroxaban specific anti-Xa activity were stable for 2 h.CONCLUSION: These results suggest that sample stability for some haemostasis assays is limited after thawing.
Databáze: OpenAIRE
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