Computational Image Analysis of Guided Acoustic Waves Enables Rheological Assessment of Sub-nanoliter Volumes
| Autor: | Jonathan M. Cooper, Steve Cohen, Aniruddha Ray, Julien Reboud, Derek Tseng, Manlio Tassieri, Aydogan Ozcan, Andriejus Demcenko, Muhammad Khalid |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Acoustics Holography General Physics and Astronomy 02 engineering and technology 010402 general chemistry 01 natural sciences Article law.invention Viscosity Rheology law Microscopy General Materials Science Thin film Biochip guided acoustic waves Relaxation (NMR) General Engineering computational image analysis Acoustic wave 021001 nanoscience & nanotechnology 0104 chemical sciences microscopy holography rheology 0210 nano-technology |
| Zdroj: | ACS Nano |
| ISSN: | 1936-086X 1936-0851 |
| Popis: | We present a method for the computational image analysis of high frequency guided sound waves based upon the measurement of optical interference fringes, produced at the air interface of a thin film of liquid. These acoustic actuations induce an affine deformation of the liquid, creating a lensing effect that can be readily observed using a simple imaging system. We exploit this effect to measure and analyze the spatiotemporal behavior of the thin liquid film as the acoustic wave interacts with it. We also show that, by investigating the dynamics of the relaxation processes of these deformations when actuation ceases, we are able to determine the liquid's viscosity using just a lens-free imaging system and a simple disposable biochip. Contrary to all other acoustic-based techniques in rheology, our measurements do not require monitoring of the wave parameters to obtain quantitative values for fluid viscosities, for sample volumes as low as 200 pL. We envisage that the proposed methods could enable high throughput, chip-based, reagent-free rheological studies within very small samples. |
| Databáze: | OpenAIRE |
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