Non-Coding RNA Sequencing of Equine Endometrium During Maternal Recognition of Pregnancy
| Autor: | Kristin M. Klohonatz, Stephen J. Coleman, Ashley D. Cameron, Ann M. Hess, Kailee J. Reed, Angela Canovas, Juan F. Medrano, Alma D. Islas-Trejo, Ted Kalbfleisch, Gerrit J. Bouma, Jason E. Bruemmer |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
endocrine system RNA Untranslated lcsh:QH426-470 animal diseases non-coding RNA maternal recognition of pregnancy embryo Reproductive health and childbirth Article 03 medical and health sciences Endometrium 0302 clinical medicine Clinical Research Pregnancy Genetics Animals Horses endometrium Genetics (clinical) reproductive and urinary physiology equine Animal urogenital system non-coding rna Untranslated lcsh:Genetics 030104 developmental biology Pregnancy Animal RNA Female sense organs pregnancy Transcriptome 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | Genes, Vol 10, Iss 10, p 821 (2019) Genes, vol 10, iss 10 Genes Volume 10 Issue 10 |
| ISSN: | 2073-4425 |
| Popis: | Maternal recognition of pregnancy (MRP) in the mare is not well defined. In a non-pregnant mare, prostaglandin F2&alpha (PGF) is released on day 14 post-ovulation (PO) to cause luteal regression, resulting in loss of progesterone production. Equine MRP occurs prior to day 14 to halt PGF production. Studies have failed to identify a gene candidate for MRP, so attention has turned to small, non-coding RNAs. The objective of this study was to evaluate small RNA (< 200 nucleotides) content in endometrium during MRP. Mares were used in a cross-over design with each having a pregnant and non-mated cycle. Each mare was randomly assigned to collection day 11 or 13 PO (n = 3/day) and endometrial biopsies were obtained. Total RNA was isolated and sequencing libraries were prepared using a small RNA library preparation kit and sequenced on a HiSeq 2000. EquCab3 was used as the reference genome and DESeq2 was used for statistical analysis. On day 11, 419 ncRNAs, representing miRNA, snRNA, snoRNA, scaRNA, and vaultRNA, were different between pregnancy statuses, but none on day 13. Equine endometrial ncRNAs with unknown structure and function were also identified. This study is the first to describe ncRNA transcriptome in equine endometrium. Identifying targets of these ncRNAs could lead to determining MRP. |
| Databáze: | OpenAIRE |
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