Coordinate expression of Ca2+-ATPase slow-twitch isoform and of beta calmodulin-dependent protein kinase in phospholamban-deficient sarcoplasmic reticulum of rabbit masseter muscle

Autor: Alessandra Pallanca, Ernesto Damiani, Alfredo Margreth, Roberta Sacchetto
Rok vydání: 2000
Předmět:
Gene isoform
Male
Calmodulin protein kinase
ATPase
Blotting
Western
Immunoblotting
Biophysics
Skeletal muscle
Calcium-Transporting ATPases
phospholamban
calmodulin protein kinase
Biochemistry
Masseter muscle
Structural Biology
Genetics
medicine
Centrifugation
Density Gradient
Animals
Phosphorylation
Molecular Biology
Phospholamban
Regulation of gene expression
biology
Chemistry
Masseter Muscle
Endoplasmic reticulum
Rabbit masseter
Calcium-Binding Proteins
Ryanodine Receptor Calcium Release Channel
Cell Biology
Molecular biology
Isoenzymes
Calmodulin dependent protein kinase
Sarcoplasmic Reticulum
medicine.anatomical_structure
Muscle Fibers
Slow-Twitch
Fluorescent Antibody Technique
Direct
Calcium-Calmodulin-Dependent Protein Kinases
biology.protein
Rabbits
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Zdroj: FEBS letters. 481(3)
ISSN: 0014-5793
Popis: Modulation of sarcoplasmic reticulum (SR) Ca2+ transport by endogenous calmodulin-dependent protein kinase II (CaM K II) involves covalent changes of regulatory protein phospholamban (PLB), as a common, but not the only mechanism, in limb slow-twitch muscles of certain mammalian species, such as the rabbit. Here, using immunofluorescent techniques in situ, and biochemical and immunological methods on the isolated SR, we have demonstrated that rabbit masseter, a muscle with a distinct embryological origin, lacks PLB. Accommodating embryological heterogeneity in the paradigm of neural-dependent expression of specific isogenes in skeletal muscle fibers, our results provide novel evidence for the differential expression in the SR of 72 kDa β components of CaM K II, together with the expression of a slow-twitch sarcoendoplasmic reticulum Ca2+-ATPase isoform, both in limb muscle and in the masseter.
Databáze: OpenAIRE
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