Endocannabinoid receptor deficiency affects maternal care and alters the dam's hippocampal oxytocin receptor and brain-derived neurotrophic factor expression
| Autor: | Moshe Gross, Andreas Zimmer, Albert Pinhasov, Michal Schechter, Aron Weller, Z. Pittel |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Cannabinoid receptor medicine.drug_class Endocrinology Diabetes and Metabolism Oxytocin Real-Time Polymerase Chain Reaction Hippocampus Cellular and Molecular Neuroscience chemistry.chemical_compound Mice Endocrinology Glucocorticoid receptor Receptor Cannabinoid CB1 Corticosterone Neurotrophic factors Internal medicine medicine Animals RNA Messenger Receptor Receptors Cannabinoid DNA Primers Brain-derived neurotrophic factor Base Sequence Behavior Animal Endocrine and Autonomic Systems Reverse Transcriptase Polymerase Chain Reaction Brain-Derived Neurotrophic Factor Oxytocin receptor Mice Inbred C57BL chemistry Mineralocorticoid Receptors Oxytocin Female Psychology |
| Zdroj: | Journal of neuroendocrinology. 25(10) |
| ISSN: | 1365-2826 |
| Popis: | Maternal care is the newborn's first experience of social interaction, and this influences infant survival, development and social competences throughout life. We recently found that postpartum blocking of the endocannabinoid receptor-1 (CB1R) altered maternal behaviour. In the present study, maternal care was assessed by the time taken to retrieve pups, pups' ultrasonic vocalisations (USVs) and pup body weight, comparing CB1R deleted (CB1R KO) versus wild-type (WT) mice. After culling on postpartum day 8, hippocampal expression of oxytocin receptor (OXTR), brain-derived neurotrophic factor (BDNF) and stress-mediating factors were evaluated in CB1R KO and WT dams. Comparisons were also performed with nulliparous (NP) CB1R KO and WT mice. Compared to WT, CB1R KO dams were slower to retrieve their pups. Although the body weight of the KO pups did not differ from the weight of WT pups, they emitted fewer USVs. This impairment of the dam-pup relationship correlated with a significant reduction of OXTR mRNA and protein levels among CB1R KO dams compared to WT dams. Furthermore, WT dams exhibited elevated OXTR mRNA expression, as well as increased levels of mineralocorticoid and glucocorticoid receptors, compared to WT NP mice. By contrast, CB1R KO dams showed no such elevation of OXTR expression, alongside lower BDNF and mineralocorticoid receptors, as well as elevated corticotrophin-releasing hormone mRNA levels, when compared to CB1R KO NP. Thus, it appears that the disruption of endocannabinoid signalling by CB1R deletion alters expression of the OXTR, apparently leading to deleterious effects upon maternal behaviour. |
| Databáze: | OpenAIRE |
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