Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration
| Autor: | Inho Jo, Yeonsil Yu, Joo Won Park, Han Su Kim, Saeyoung Park, Sung Chul Jung, Jae Kwang Kim, Yoonyoung Choi, Young Bin Hong, Kyung Ha Ryu, Byung Ok Choi, Namhee Jung, Geon Kwak, Hyun Ho Choi Park |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine tonsil-derived mesenchymal stem cells Schwann cell differentiation peripheral nerve regeneration Cellular differentiation Palatine Tonsil Nerve guidance conduit Gene Expression lcsh:Chemistry Mice 0302 clinical medicine Dorsal root ganglion Peripheral Nerve Injuries Ganglia Spinal Child lcsh:QH301-705.5 Spectroscopy Neurons integumentary system Cell Differentiation General Medicine Sciatic nerve injury Sciatic Nerve Computer Science Applications Cell biology medicine.anatomical_structure Transplantation Heterologous Nerve Tissue Proteins Biology Article Catalysis Inorganic Chemistry 03 medical and health sciences medicine Animals Humans Physical and Theoretical Chemistry Molecular Biology Tonsillectomy Regeneration (biology) Organic Chemistry Mesenchymal stem cell Mesenchymal Stem Cells Recovery of Function medicine.disease Coculture Techniques Nerve Regeneration Mice Inbred C57BL Transplantation 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 nervous system Immunology Schwann Cells Biomarkers 030217 neurology & neurosurgery |
| Zdroj: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(17): 11 International Journal of Molecular Sciences, Vol 17, Iss 11, p 1867 (2016) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 17; Issue 11; Pages: 1867 |
| ISSN: | 1422-0067 |
| Popis: | Schwann cells (SCs), which produce neurotropic factors and adhesive molecules, have been reported previously to contribute to structural support and guidance during axonal regeneration; therefore, they are potentially a crucial target in the restoration of injured nervous tissues. Autologous SC transplantation has been performed and has shown promising clinical results for treating nerve injuries and donor site morbidity, and insufficient production of the cells have been considered as a major issue. Here, we performed differentiation of tonsil-derived mesenchymal stem cells (T-MSCs) into SC-like cells (T-MSC-SCs), to evaluate T-MSC-SCs as an alternative to SCs. Using SC markers such as CAD19, GFAP, MBP, NGFR, S100B, and KROX20 during quantitative real-time PCR we detected the upregulation of NGFR, S100B, and KROX20 and the downregulation of CAD19 and MBP at the fully differentiated stage. Furthermore, we found myelination of axons when differentiated SCs were cocultured with mouse dorsal root ganglion neurons. The application of T-MSC-SCs to a mouse model of sciatic nerve injury produced marked improvements in gait and promoted regeneration of damaged nerves. Thus, the transplantation of human T-MSCs might be suitable for assisting in peripheral nerve regeneration. |
| Databáze: | OpenAIRE |
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