Action of Vitamin D and the Receptor, VDRa, in Calcium Handling in Zebrafish (Danio rerio)

Autor: Feng-Chun Ding, Pung-Pung Hwang, Che-Hsien Su, Chia-Hao Lin, Deng-Yu Tseng
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Anatomy and Physiology
Morpholino
lcsh:Medicine
Calcitriol receptor
Integrative Physiology
Molecular Cell Biology
Vitamin D
lcsh:Science
Zebrafish
Gene knockdown
Multidisciplinary
biology
Reverse Transcriptase Polymerase Chain Reaction
Animal Models
Immunohistochemistry
Cell biology
Gene Knockdown Techniques
embryonic structures
Sodium-Potassium-Exchanging ATPase
Research Article
Signal Transduction
Molecular Sequence Data
chemistry.chemical_element
TRPV Cation Channels
Endocrine System
Calcium
Real-Time Polymerase Chain Reaction
Signaling Pathways
Model Organisms
CYP24A1
Vitamin D and neurology
Calcium-Mediated Signal Transduction
Animal Physiology
Animals
Amino Acid Sequence
RNA
Messenger
Biology
DNA Primers
Endocrine Physiology
Base Sequence
Sequence Homology
Amino Acid
Calcium channel
lcsh:R
Zebrafish Proteins
biology.organism_classification
Molecular biology
Hormones
chemistry
Receptors
Calcitriol
lcsh:Q
Zoology
Zdroj: PLoS ONE
PLoS ONE, Vol 7, Iss 9, p e45650 (2012)
ISSN: 1932-6203
Popis: The purpose of the present study was to use zebrafish as a model to investigate how vitamin D and its receptors interact to control Ca(2+) uptake function. Low-Ca(2+) fresh water stimulated Ca(2+) influx and expressions of epithelial calcium channel (ecac), vitamin D-25-hydroxylase (cyp2r1), vitamin D receptor a (vdra), and vdrb in zebrafish. Exogenous vitamin D increased Ca(2+) influx and expressions of ecac and 25-hydroxyvitamin D(3)-24-hydroxylase (cyp24a1), but downregulated 1α-OHase (cyp27b1) with no effects on other Ca(2+) transporters. Morpholino oligonucleotide knockdown of VDRa, but not VDRb, was found as a consequence of calcium uptake inhibition by knockdown of ecac, and ossification of vertebrae is impaired. Taken together, vitamin D-VDRa signaling may stimulate Ca(2+) uptake by upregulating ECaC in zebrafish, thereby clarifying the Ca(2+)-handling function of only a VDR in teleosts. Zebrafish may be useful as a model to explore the function of vitamin D-VDR signaling in Ca(2+) homeostasis and the related physiological processes in vertebrates.
Databáze: OpenAIRE
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