Higher prevalence of multidrug-resistant extended-spectrum β-lactamases producing Escherichia coli in unorganized pig farms compared to organized pig farms in Mizoram, India
| Autor: | P. K. Subudhi, S Bandyopadhayay, Parimal Roychoudhury, Hosterson Kylla, Tapan Kumar Dutta, Rajkumari Mandakini, Indranil Samanta |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
pig Veterinary medicine Tetracycline medicine.drug_class Sulfafurazole 030106 microbiology Cephalosporin Population multidrug-resistant Biology SF1-1100 03 medical and health sciences extended-spectrum β-lactamases Ampicillin SF600-1100 medicine education education.field_of_study General Veterinary india biochemical phenomena metabolism and nutrition Animal culture Multiple drug resistance Ciprofloxacin 030104 developmental biology mizoram escherichia coli medicine.drug Piperacillin Research Article |
| Zdroj: | Veterinary World, Vol 13, Iss 12, Pp 2752-2758 (2020) Veterinary World |
| ISSN: | 2231-0916 0972-8988 |
| Popis: | Aim: The present study was conducted to record the prevalence of multidrug-resistant (MDR), extended-spectrum β-lactamases (ESBLs) producing Escherichia coli from pig population of organized and unorganized farms of Mizoram and to record the presence of ESBLs, non-ESBLs, and integrons. Materials and Methods: Fecal samples were collected from pigs under organized (n=40) and unorganized (n=58) farms of Mizoram. Samples were processed for isolation and identification of E. coli by conventional techniques, BD Phoenix™ automated bacterial system, and polymerase chain reaction (PCR) based confirmatory test. All the isolates were subjected to antimicrobial sensitivity test by disk diffusion assay and ESBLs production by double-disk synergy test (DDST). The ESBLs producing isolates were subjected to PCR for determination of ESBLs genes and all the isolates were screened for non-ESBLs genes and integrons by PCR. Results: A total of 258 E. coli was isolated and identified from organized (n=120) and unorganized farms (n=138). Majority of the E. coli isolates exhibited high level of resistance against amoxicillin (Ax) (81.78%), cefalexin (85.42%), co-trimoxazole (50.78%), sulfafurazole (69.38%), tetracycline (65.89%), and trimethoprim (TR) (51.94%). Statistically highly significant (p |
| Databáze: | OpenAIRE |
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