Polyphenol oxidase from hybrid poplar. Cloning and expression in response to wounding and herbivory
| Autor: | C. Peter Constabel, Joseph J. Patton, Mary E. Christopher, Lynn Yip |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Populus trichocarpa
DNA Complementary Insecta Physiology Molecular Sequence Data Plant Science Cyclopentanes Acetates Polyphenol oxidase Trees chemistry.chemical_compound Salicaceae Gene Expression Regulation Plant Botany Gene expression Genetics Animals Amino Acid Sequence Oxylipins RNA Messenger Cloning Molecular Catechol oxidase Crosses Genetic Hybrid Methyl jasmonate biology integumentary system Base Sequence Tent caterpillar fungi food and beverages biology.organism_classification Blotting Northern Blotting Southern chemistry RNA Plant Enzyme Induction biology.protein Catechol Oxidase Research Article |
| Zdroj: | Plant physiology. 124(1) |
| ISSN: | 0032-0889 |
| Popis: | The inducible expression of polyphenol oxidase (PPO), a presumed antiherbivore enzyme, was examined in hybrid poplar (Populus trichocarpa × Populus deltoides). Following mechanical wounding simulating insect damage, PPO activity increased dramatically in wounded and unwounded leaves on wounded plants beginning at 24 and 48 h, respectively. A hybrid poplar PPO cDNA was isolated and its nucleotide sequence determined. On northern blots, PPO transcripts were detected within 8 h of wounding, and reached peak levels at 16 and 24 h in wounded and unwounded leaves, respectively. Methyl jasmonate spray and feeding by forest tent caterpillar also induced PPO expression. The induction of PPO was strongest in the youngest four leaves, which were generally avoided by caterpillars in free feeding experiments. This wound- and herbivore-induced expression of PPO in hybrid poplar supports the defensive role of this protein against insect pests. |
| Databáze: | OpenAIRE |
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