Microbial colonization of pancreatic duct stents: a prospective analysis
| Autor: | Philipp Schenk, Jochen Schneider, Bruno Neu, Roland M. Schmid, Franziska Römmler, Susanne Feihl, Stefanie Forkl, Nina Wantia, Andreas Obermeier, Hana Algül, Stefan von Delius, Andreas Weber, Julia Fremd, Monther Bajbouj |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male medicine.medical_specialty Time Factors Endocrinology Diabetes and Metabolism medicine.medical_treatment Gastroenterology Microbiology Sonication Young Adult Endocrinology Internal medicine Internal Medicine medicine Microbial colonization Humans Clinical significance Prospective Studies Prospective cohort study Device Removal Aged Pancreatic duct Aged 80 and over Cholangiopancreatography Endoscopic Retrograde Hepatology biology Bacteria Biofilm Pancreatic Ducts Stent Middle Aged equipment and supplies biology.organism_classification Enterobacteriaceae medicine.anatomical_structure Biofilms Equipment Contamination Female Stents |
| Zdroj: | Pancreas. 44(5) |
| ISSN: | 1536-4828 |
| Popis: | OBJECTIVE The aim of the study was to analyze the microbial colonization rate as well as the spectrum and number of microorganisms in relation to the indwelling time of pancreatic stents. METHODS Forty pancreatic stents were prepared according to a standardized protocol and subsequently sonicated to optimize bacterial release from the biofilm on the stents. RESULTS Two hundred forty-six microorganisms were identified. Thirty-nine of 40 stents were colonized with microorganisms. Aerobic gram-positive microorganisms (106/246 [43%]) accounted for the greatest proportion. The predominant microorganisms were Streptococcus species (46/246 [19%]), which were isolated from 27 (68%) of 40 stents. Stents with a short indwelling time (3-13 days) were mainly colonized with aerobic gram-positive bacteria (82%) and Candida species (63%). In contrast, anaerobes (P < 0.01, 69% vs 18%) and aerobic gram-negative microorganisms (P < 0.01, 93% vs 45%) such as Enterobacteriaceae (P < 0.01, 86% vs 27%) were significantly more present on stents with a long indwelling time (29-93 days), compared with stents with a short indwelling time. CONCLUSIONS Microbial analysis of pancreatic duct stents revealed a very high colonization rate. Furthermore, the spectrum and number of microorganisms altered with the indwelling time of the stent. However, clinical relevance of our findings remains unclear. |
| Databáze: | OpenAIRE |
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