Live Imaging of Intracranial Lymphatics in the Zebrafish
| Autor: | Daniel Castranova, Van N. Pham, Marina Venero Galanternik, Brant M. Weinstein, Bakary Samasa, Hyun Min Jung |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Physiology Fluid homeostasis Neutrophils ved/biology.organism_classification_rank.species Vascular Endothelial Growth Factor C Biology Article Lymphatic System Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine Meninges Interstitial fluid Live cell imaging medicine Animals Lymphangiogenesis Model organism Zebrafish 030304 developmental biology Lymphatic Vessels 0303 health sciences Microscopy Confocal ved/biology Optical Imaging Brain biology.organism_classification 030104 developmental biology Lymphatic system medicine.anatomical_structure Vascular network Neutrophil Infiltration Lymphatic function Cardiology and Cardiovascular Medicine Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Circ Res |
| ISSN: | 1524-4571 |
| Popis: | Rationale: The recent discovery of meningeal lymphatics in mammals is reshaping our understanding of fluid homeostasis and cellular waste management in the brain, but visualization and experimental analysis of these vessels is challenging in mammals. Although the optical clarity and experimental advantages of zebrafish have made this an essential model organism for studying lymphatic development, the existence of meningeal lymphatics has not yet been reported in this species. Objective: Examine the intracranial space of larval, juvenile, and adult zebrafish to determine whether and where intracranial lymphatic vessels are present. Methods and Results: Using high-resolution optical imaging of the meninges in living animals, we show that zebrafish possess a meningeal lymphatic network comparable to that found in mammals. We confirm that this network is separate from the blood vascular network and that it drains interstitial fluid from the brain. We document the developmental origins and growth of these vessels into a distinct network separated from the external lymphatics. Finally, we show that these vessels contain immune cells and perform live imaging of immune cell trafficking and transmigration in meningeal lymphatics. Conclusions: This discovery establishes the zebrafish as a important new model for experimental analysis of meningeal lymphatic development and opens up new avenues for probing meningeal lymphatic function in health and disease. |
| Databáze: | OpenAIRE |
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