Layer‐by‐layer bioassembly of poly(lactic) acid membranes loaded with coculture of HBMSCs and EPCs improves vascularization in vivo

Autor: Jean-Christophe Fricain, Raphaël Devillard, Reine Bareille, Maxime Seimbille, Vera Guduric, Noélie B. Thebaud, Ognjan Luzanin, Joanna Babilotte, Damien Le Nihouannen, Sylvie Rey, Robin Siadous, Sylvain Catros
Přispěvatelé: Bioingénierie tissulaire (BIOTIS), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Bordeaux (UB), Faculty of Sciences [University of Novi Sad], University of Novi Sad, Chassande, Olivier
Rok vydání: 2019
Předmět:
Male
Stromal cell
Materials science
Polyesters
layer-by-layer
[SDV]Life Sciences [q-bio]
0206 medical engineering
Biomedical Engineering
Neovascularization
Physiologic
Biocompatible Materials
02 engineering and technology
Mesenchymal Stem Cell Transplantation
Endothelial cell differentiation
Biomaterials
Mice
chemistry.chemical_compound
vascularization
Tissue engineering
Animals
Humans
Progenitor cell
Cells
Cultured
Endothelial Progenitor Cells
Tissue Scaffolds
biofabrication
Metals and Alloys
Cell Differentiation
Membranes
Artificial
Mesenchymal Stem Cells
021001 nanoscience & nanotechnology
020601 biomedical engineering
Coculture Techniques
Lactic acid
[SDV] Life Sciences [q-bio]
in vivo
Membrane
chemistry
tissue engineering
Printing
Three-Dimensional
Ceramics and Composites
Biophysics
Alkaline phosphatase
0210 nano-technology
Biofabrication
Zdroj: Journal of Biomedical Materials Research Part A
Journal of Biomedical Materials Research Part A, Wiley, 2019, 107 (12), pp.2629-2642. ⟨10.1002/jbm.a.36769⟩
ISSN: 1552-4965
1549-3296
DOI: 10.1002/jbm.a.36769
Popis: International audience; Layer-by-layer (LBL) BioAssembly method was developed to enhance the control of cell distribution within 3D scaffolds for tissue engineering applications. The objective of this study was to evaluate in vivo the development of blood vessels within LBL bioassembled membranes seeded with human primary cells, and to compare it to cellularized massive scaffolds. Poly(lactic) acid (PLA) membranes fabricated by fused deposition modeling were seeded with monocultures of human bone marrow stromal cells or with cocultures of these cells and endothelial progenitor cells. Then, four cellularized membranes were assembled in LBL constructs. Early osteoblastic and endothelial cell differentiation markers, alkaline phosphatase, and von Willebrand's factor, were expressed in all layers of assemblies in homogenous manner. The same kind of LBL assemblies as well as cellularized massive scaffolds was implanted subcutaneously in mice. Human cells were observed in all scaffolds seeded with cells, but not in the inner parts of massive scaffolds. There were significantly more blood vessels observed in LBL bioassemblies seeded with cocultures compared to all other samples. LBL bioassembly of PLA membranes seeded with a coculture of human cells is an efficient method to obtain homogenous cell distribution and blood vessel formation within the entire volume of a 3D composite scaffold.
Databáze: OpenAIRE
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