Retention modeling of gradient elutions: Application of iso-to-grad approach for LC systems with dual-species eluent
| Autor: | Tomislav Bolanča, Marko Rogošić, Mirjana Novak Stankov, Matija Cvetnić, Šime Ukić, Vladimir Stankov |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Integral model
Hplc analysis Chromatography 010405 organic chemistry Chemistry Elution 010401 analytical chemistry Organic Chemistry Clinical Biochemistry iso-to-grad information transfer liquid chromatography method development dual-species eluent 01 natural sciences Biochemistry High-performance liquid chromatography 0104 chemical sciences Analytical Chemistry Column chromatography Dual species Gradient elution |
| Popis: | Development of new chromatographic method is often time-consuming and may be shortened by mathematical modeling. Unfortunately, many of the models are limited for predefined elution programs only. The integral model uses iso-to-grad approach to transfer information from isocratic to gradient elution without such limitations, which was successfully tested for IC and HPLC using single-species eluents. Here its applicability was tested on systems using more complex eluents. An HPLC analysis of emerging contaminants using methanol/acetonitrile/water eluent was performed. Four different integral models were developed and validated. The models showed good predictive ability. |
| Databáze: | OpenAIRE |
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