Antifungal activities of azole agents against the Malassezia species
| Autor: | Orionalda de Fátima Lisboa Fernandes, Karla Carvalho Miranda, Carolina Rodrigues Costa, Xisto Sena Passos, Crystiane Rodrigues de Araujo, Maria do Rosário Rodrigues Silva |
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| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Azoles Antifungal Agents Itraconazole Microbial Sensitivity Tests In Vitro Techniques Microbiology Minimum inhibitory concentration Tinea Versicolor medicine Humans Pharmacology (medical) Fluconazole Voriconazole chemistry.chemical_classification Malassezia integumentary system biology Broth microdilution General Medicine Triazoles biology.organism_classification Infectious Diseases Ketoconazole Pyrimidines chemistry Azole medicine.drug |
| Zdroj: | International journal of antimicrobial agents. 29(3) |
| ISSN: | 0924-8579 |
| Popis: | In this paper, we identified 95 Malassezia isolates by morphological and biochemical criteria and assessed the in vitro activity of fluconazole, itraconazole, ketoconazole and voriconazole by broth microdilution against these species using slightly modified Leeming-Notman medium. The Malassezia isolates were identified as M. furfur (74), M. sympodialis (11), M. obtusa (8) and M. globosa (2). The modified Leeming-Notman medium used for susceptibility testing allowed good growth of Malassezia spp. Visual reading of the minimal inhibitory concentration (MIC) was readily achieved until Day 5 of incubation at 32 degrees C. Although high MIC values of 16 microg/mL for fluconazole were observed in 9.5% of Malassezia isolates, in general these microorganisms were susceptible to all drugs studied. Interestingly, one M. globosa isolate showed high MIC values for voriconazole, itraconazole and fluconazole. For the 95 strains, the MIC ranges were0.03-4 microg/mL for ketoconazole,0.03 to16 microg/mL for voriconazole,0.125 to64 microg/mL for fluconazole and0.03-16 microg/mL for itraconazole. In summary, the good reproducibility and visual readings obtained using modified Leeming-Notman medium suggest that this medium should be proposed for antifungal testing of drugs against Malassezia spp. |
| Databáze: | OpenAIRE |
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