HBV genotypes prevalence, precore and basal core mutants in Morocco
| Autor: | Fatiha Lazar, Abdelouahad El Malki, Elmostafa El Fahime, Marouane Melloul, Warda Baha, My Mustapha Ennaji, Abdelouaheb Bennani |
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| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Adult Male Hepatitis B virus Adolescent Genes Viral Genotype Population Mutant DNA Mutational Analysis Biology medicine.disease_cause Microbiology Polymerase Chain Reaction law.invention law Multiplex polymerase chain reaction Genetics medicine Prevalence Humans education Molecular Biology Genotyping Ecology Evolution Behavior and Systematics Polymerase chain reaction Phylogeny Aged education.field_of_study Chi-Square Distribution Hepatitis B Middle Aged medicine.disease Virology Molecular biology Morocco Infectious Diseases DNA Viral Mutation Female |
| Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 12(6) |
| ISSN: | 1567-7257 |
| Popis: | The study of hepatitis B virus (HBV) genomic heterogeneity has become a major issue in investigations aimed at understanding the relationship between HBV mutants and the wide spectrum of clinical and pathological conditions associated with HBV infection. The objective of the current study was to find out the pattern of HBV genotypes circulating in Morocco and to investigate the precore (PC) and basal core promoter (BCP) mutants' status in Moroccan chronic hepatitis B patients. Viral genotypes were determined in 221 chronic carriers using INNO-LiPA HBV assay and hemi-nested PCR. Phylogenetic analysis was performed in 70 samples, and multiplex PCR method was used to confirm some genotyping results. PC and CP mutants were determined using Inno-Lipa. All isolates were successfully genotyped. The genotype distribution was D in 90.45% of cases, A (5.9%), E (1 case), and mixed genotypes (5 A/D and 2 D/F) in 3.17% patients. HBV carried in the HBV/D samples could be assigned to D7 (63.3%), D1 (32.7%) and 2% of strains to each D4 and D5, all HBV/A belonged to A2 subgenotype and HBV/E strain could not be sub-genotyped. In 70 studied strains, HBV mutants were detected in 88.6% of cases; PC mutants were detected in (40%) of patients and 21.5% present a mixture of wild type and G1896A mutation. BCP mutants were observed in 65.7% of cases, 22.9% were found to have the T1762/1764A double mutation, 18.6% had A1762/1764T mutation and 22.9% of patients showed the A1762T/G1764A double mutation with either A1762T/G1764T mutation. Co-infection by PC and BCP mutants was detected in 52.9% of cases. Movement from place to place most likely shapes the observed genotype distribution and consequent prevalence of genotypes other than A2 or D7 in this population. High circulation of PC and BCP mutants is common in chronic hepatitis B infection in Morocco. |
| Databáze: | OpenAIRE |
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