Effects of FSH on the expression of receptors for oocyte-secreted factors and members of the EGF-like family during in vitro maturation in cattle

Autor: E. S. Caixeta, Christopher A. Price, Jose Buratini, M. F. Machado, P. Ripamonte
Rok vydání: 2012
Předmět:
endocrine system
medicine.medical_specialty
Sus scrofa
Growth differentiation factor-9
Biology
Ligands
Amphiregulin
Epiregulin
Andrology
Follicle-stimulating hormone
Endocrinology
Epidermal growth factor
Internal medicine
Genetics
medicine
Animals
Protein Isoforms
Betacellulin
Receptor
Fibroblast Growth Factor
Type 2
Receptor
Molecular Biology
Bone Morphogenetic Protein Receptors
Type I
Cells
Cultured
Cell Proliferation
Glycoproteins
Cumulus Cells
Epidermal Growth Factor
Gene Expression Regulation
Developmental
Fertility Agents
Female
Coculture Techniques
In vitro maturation
In Vitro Oocyte Maturation Techniques
Reproductive Medicine
Oocytes
Intercellular Signaling Peptides and Proteins
Animal Science and Zoology
Cattle
Female
Follicle Stimulating Hormone
hormones
hormone substitutes
and hormone antagonists
Abattoirs
Developmental Biology
Biotechnology
Zdroj: Reproduction, fertility, and development. 25(6)
ISSN: 1031-3613
Popis: FSH induces expansion of bovine cumulus–oocyte complexes (COCs) in cattle, which can be enhanced by oocyte-secreted factors (OSFs). In this study it was hypothesised that FSH stimulates COC expansion in part from direct stimulation of the epidermal growth factor (EGF)-like ligands amphiregulin (AREG), epiregulin (EREG) and betacellulin (BTC), but also in part through regulation of OSFs or their receptors in cumulus cells. Bovine COCs were cultured in defined medium with graded doses of FSH. In the absence of FSH, COCs did not expand. FSH caused cumulus expansion, and increased the abundance of AREG and EREG mRNA in a time- and dose-dependent manner, but decreased BTC mRNA levels. FSH had modest stimulatory effects on the levels of mRNA encoding the bone morphogenetic protein 15 (BMP15) receptor, BMPR1B, in cumulus cells, but did not alter mRNA expression of the growth and differentiation factor 9 (GDF9) receptor, TGFBR1. More interestingly, FSH dramatically stimulated levels of mRNA encoding two receptors for fibroblast growth factors (FGF), FGFR2C and FGFR3C, in cumulus cells. FSH also stimulated mRNA expression of FGFR1B, but not of FGFR2B in cumulus cells. Based on dose-response studies, FGFR3C was the receptor most sensitive to the influence of FSH. This study demonstrates that FSH stimulates the expression of EGF-like factors in bovine cumulus cells, and provides evidence that FSH differently regulates the expression of distinct receptors for OSFs in cumulus cells.
Databáze: OpenAIRE
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