Electric field-induced changes in biomechanical properties in human dermal fibroblasts and a human skin equivalent
| Autor: | Donggerami Moon, Kyung Sook Kim, Jihui Jang, Sangwoo Kwon, Jun Bae Lee, Se Jik Han, Moon Young Park, Minjoo Noh |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cell
Stimulation Human skin Dermatology Microscopy Atomic Force 01 natural sciences Collagen fibril 010309 optics 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Electricity Electric field 0103 physical sciences medicine Humans Elasticity (economics) skin and connective tissue diseases Actin Cells Cultured Skin integumentary system Chemistry Atomic force microscopy Fibroblasts Elasticity Extracellular Matrix medicine.anatomical_structure Biophysics sense organs Collagen Biomarkers |
| Zdroj: | Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)REFERENCES. 26(6) |
| ISSN: | 1600-0846 |
| Popis: | Purpose An electric field (EF) can be used to change the mechanical properties of cells and skin tissues. We demonstrate EF-induced elasticity changes in human dermal fibroblasts (HDFs) and a human skin equivalent and identify the underlying principles related to the changes. Methods HDFs and human skin equivalent were stimulated with electric fields of 1.0 V/cm. Change in cellular elasticity was determined by using atomic force microscopy. Effects of EF on the biomechanical and chemical properties of a human skin equivalent were analyzed. In cells and tissues, the effects of EF on biomarkers of cellular elasticity were investigated at the gene and protein levels. Results In HDFs, the cellular elasticity was increased and the expression of biomarkers of cellular elasticity was regulated by the EF. Expression of the collagen protein in the human skin equivalent was changed by EF stimulation; however, changes in density and microstructure of the collagen fibrils were not significant. The viscoelasticity of the human skin equivalent increased in response to EF stimulation, but molecular changes were not observed in collagen. Conclusions Elasticity of cells and human skin equivalent can be regulated by electrical stimulation. Especially, the change in cellular elasticity was dependent on cell age. |
| Databáze: | OpenAIRE |
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