Sweet Chestnut (Castanea sativa Mill.) Bark Extract: Cardiovascular Activity and Myocyte Protection against Oxidative Damage

Autor: Tullia Gallina Toschi, Marco Malaguti, Alberto Chiarini, Monia Lenzi, Carmela Fimognari, Silvana Hrelia, Patrizia Comandini, Roberta Budriesi, Pierfranco Ioan, Matteo Micucci
Přispěvatelé: A. Chiarini, M. Micucci, M. Malaguti, R. Budriesi, P. Ioan, M. Lenzi, C. Fimognari, T. Gallina Toschi, P. Comandini, S. Hrelia
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Chronotropic
HPLC-DAD-MS analysi
Aging
CARDIOVASCULAR FUNCTIONS
Antioxidant
medicine.medical_treatment
HYDROLYSABLE TANNINS
Animals
Animals
Newborn
Atrial Function
Cardiotonic Agents
Cell Survival
Cells
Cultured
Fagaceae
Guinea Pigs
In Vitro Techniques
Myocytes
Cardiac
Oxidative Stress
Papillary Muscles
Plant Bark
Plant Extracts
Rats
Reactive Oxygen Species
Receptors
Adrenergic
Receptors
Cholinergic
medicine.disease_cause
Biochemistry
Receptors
Myocyte
Cholinergic
Cultured
lcsh:Cytology
NATURAL EXTRACT OF CHESTNUT
General Medicine
isolated guinea pig tissue
Adrenergic
Castanea Sativa Mill
neonatal rat cardiomyocyte
Cardiac
Research Article
medicine.medical_specialty
Adrenergic receptor
Article Subject
Cells
Biology
complex mixtures
Guinea pig
CARDIOPROTECTION
Internal medicine
medicine
Viability assay
lcsh:QH573-671
Myocytes
Cell Biology
Newborn
Endocrinology
ANTIOXIDANT ACTIVITY
Oxidative stress
Zdroj: Oxidative Medicine and Cellular Longevity, Vol 2013 (2013)
Oxidative Medicine and Cellular Longevity
ISSN: 1942-0994
1942-0900
Popis: This work was aimed at evaluating the cardioprotective effects ofCastanea sativaMill. (CSM) bark extract characterized in its phenolic composition by HPLC-DAD-MS analysis. The study was performed using primary cultures of neonatal rat cardiomyocytes to investigate the antioxidant and cytoprotective effects of CSM bark extract and isolated guinea pig left and right atria, left papillary muscle, and aorta to evaluate its direct effect on cholinergic and adrenergic response. In cultured cardiomyocytes the CSM bark extract reduced intracellular reactive oxygen species formation and improved cell viability following oxidative stress in dose-dependent manner. Moreover, the extract decreased the contraction induced by noradrenaline (1 μM) in guinea pig aortic strips and induced transient negative chronotropic and positive inotropic effects without involvement of cholinergic or adrenergic receptors in the guinea pig atria. Our results indicate that CSM bark extract exhibits antioxidant activity and might induce cardioprotective effect.
Databáze: OpenAIRE
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