Infecção de Ehrlichia spp. em gatos domésticos da Grande Rio de Janeiro, sudeste do Brasil
| Autor: | Marcos Rogério André, Juliana Macedo Raimundo, Cristiane Divan Baldani, Huarrisson Azevedo Santos, Maristela Peckle Peixoto, Raisa Braul Rodrigues, Rosangela Zacarias Machado, Andresa Guimarães |
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| Přispěvatelé: | Universidade Federal Rural do Rio de Janeiro - UFRRJ, Universidade Federal Fluminense (UFF), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Anemia 030231 tropical medicine Cat Diseases Polymerase Chain Reaction law.invention Microbiology Serology Feline 03 medical and health sciences 0302 clinical medicine law medicine Animals Fluorescent Antibody Technique Indirect Polymerase chain reaction lcsh:SF1-1100 CATS General Veterinary biology hematology Ehrlichia Ehrlichiosis Hematology 030108 mycology & parasitology biology.organism_classification medicine.disease Antibodies Bacterial nested PCR Canis ehrlichiosis Ehrlichiosis (canine) Cats Female Parasitology lcsh:Animal culture Nested polymerase chain reaction Brazil Nested PCR IFA |
| Zdroj: | Revista Brasileira de Parasitologia Veterinária, Vol 28, Iss 1, Pp 180-185 (2019) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1984-2961 0103-846X |
| Popis: | Made available in DSpace on 2019-10-06T16:23:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01. Added 1 bitstream(s) on 2019-10-09T18:35:21Z : No. of bitstreams: 1 S1984-29612019000100180.pdf: 1026926 bytes, checksum: 3554892a2d6c8d0aed35572f3b2c37fc (MD5) Ehrlichiosis is caused by agents belonging to Ehrlichia genus. Despite the frequent reports on the serological and molecular detection of E. canis in dogs in Brazil, there is scant data on ehrlichiosis in brazilian cats. This study aimed at investigating the occurrence of Ehrlichia spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. We searched for IgG antibodies against E. canis on blood samples of 216 cats by Indirect Fluorescence Assay (IFA). Additionally, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting E. canis-16S rRNA and dsb gene, respectively. Fifty-seven (26.4%) cats were seropositive for Ehrlichia spp. by IFA. Ehrlichia spp.-16S rRNA gene fragments were detected in 3 cats (1.4%). Although the obtained 16S rRNA sequences showed 99 to 100% identity with E. canis, cats were negative in qPCR. Anemia, thrombocytopenia, leukocytosis, left shift neutrophil and hyperproteinemia were observed. Anemia was statistically associated with seropositivity to E. canis and kittens showed lower positivity rates (p |
| Databáze: | OpenAIRE |
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