Glycosaminoglycan Metabolism and Cytokine Release in Normal and Otosclerotic Human Bone Cells Interleukin-1 Treated
| Autor: | Giordano Stabellini, Venti G, Maria Bodo, Ennio Becchetti, Emilio Donti, Paolo Carinci, Gaetano Paludetti, Monica Giammarioli |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Glycoside Hydrolases
medicine.medical_treatment Alpha (ethology) Biochemistry Bone and Bones Bone remodeling Extracellular matrix Paracrine signalling Rheumatology Bone cell medicine Humans Orthopedics and Sports Medicine Autocrine signalling Molecular Biology Cells Cultured Glycosaminoglycans Interleukin-6 Chemistry Interleukin Cell Biology Cell biology Enzyme Activation Otosclerosis Cytokine Cytokines Female Interleukin-1 |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1607-8438 0300-8207 |
| Popis: | Glycosaminoglycans (GAGs), normal components of the extracellular matrix (ECM), and the glycosidases, that degrade them, play a key role in the bone remodelling process. The effects of interleukin-1 alpha (IL-1 alpha) on GAG metabolism in normal and otosclerotic human bone cells as well as its capacity to modulate IL-1 alpha, IL-1 beta and IL-6 secretion in both populations was analyzed. The amount of radiolabeled GAGs was lower in otosclerotic than in normal bone cells. IL-1 alpha reduced newly synthesized cellular and extracellular GAGs in normal cells, but only those of the cellular compartment in otosclerotic bone cells. It depressed heparan sulphate (HS) more in normal cells and chondroitin sulphate (CS) more in otosclerotic bone cells. The HA/total sulphated GAG ratio was shifted in favour of the latter in otosclerotic cells, whereas the opposite effect was seen after IL-1 alpha treatment. There was little difference in the beta-D-glucuronidase levels of the normal and pathological cells, while beta-N-acetyl-D-glucosaminidase was significantly increased in otosclerotic bone cells. As the activity of neither enzyme was modified by treatment with IL-1 alpha, the cytokine seems to exert its influences on GAG synthesis rather than on the degradation process. IL-1 alpha, IL-1 beta and IL-6 secretion was markedly higher in otosclerotic cells. IL-1 alpha modulated the secretion of each interleukin differently, thus resulting in a cytokine cascade that may act in autocrine/paracrine manner on target cells. The authors suggest that changes in the cytokine network may have a specific, yet still unknown, role during normal and pathological osteogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|