Dynamic modulation of activity in cerebellar nuclei neurons during pavlovian eyeblink conditioning in mice
| Autor: | Xiaolu Wang, Michiel M. ten Brinke, Shane A. Heiney, Jacob Bakermans, Henk-Jan Boele, Javier F. Medina, Zhenyu Gao, Martina Proietti-Onori, Chris I. De Zeeuw |
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| Přispěvatelé: | Netherlands Institute for Neuroscience (NIN), Neurosciences |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Mouse QH301-705.5 Science Stimulation cerebellar nuclei eyeblink conditioning Optogenetics Biology General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine Journal Article medicine Animals Biology (General) Latency (engineering) Neurons General Immunology and Microbiology General Neuroscience General Medicine Conditioning Eyelid 030104 developmental biology medicine.anatomical_structure nervous system Dynamic modulation Eyeblink conditioning Cerebellar cortex Facilitation cortical subcortical communication Medicine Nucleus Neuroscience 030217 neurology & neurosurgery Research Article |
| Zdroj: | eLife, 6:e28132. eLife Sciences Publications eLife eLife, Vol 6 (2017) |
| ISSN: | 2050-084X |
| DOI: | 10.7554/elife.28132 |
| Popis: | While research on the cerebellar cortex is crystallizing our understanding of its function in learning behavior, many questions surrounding its downstream targets remain. Here, we evaluate the dynamics of cerebellar interpositus nucleus (IpN) neurons over the course of Pavlovian eyeblink conditioning. A diverse range of learning-induced neuronal responses was observed, including increases and decreases in activity during the generation of conditioned blinks. Trial-by-trial correlational analysis and optogenetic manipulation demonstrate that facilitation in the IpN drives the eyelid movements. Adaptive facilitatory responses are often preceded by acquired transient inhibition of IpN activity that, based on latency and effect, appear to be driven by complex spikes in cerebellar cortical Purkinje cells. Likewise, during reflexive blinks to periocular stimulation, IpN cells show excitation-suppression patterns that suggest a contribution of climbing fibers and their collaterals. These findings highlight the integrative properties of subcortical neurons at the cerebellar output stage mediating conditioned behavior. |
| Databáze: | OpenAIRE |
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