The plant specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis
| Autor: | Daniël Van Damme, Yonsheng Guan, Arp Schnittger, Sascha Biedermann, Hirofumi Harashima, Peter Doerner, Annika K. Weimer, Maren Heese, Csaba Koncz, Masaaki Umeda, Gaëtan Pochon, Keiko Sugimoto, Julia Foreman, Farshad Roodbarkelari, Naoki Takahashi |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell division DNA repair DNA damage CDK RAD51 Plant Biology Cell Cycle Proteins DNA-DAMAGE RESPONSE homologous recombination DOUBLE-STRAND BREAKS General Biochemistry Genetics and Molecular Biology Article END RESECTION 03 medical and health sciences chemistry.chemical_compound cyclin Cyclin-dependent kinase Molecular Biology General Immunology and Microbiology biology General Neuroscience GAMMA-RAYS DNA Replication Repair & Recombination Biology and Life Sciences DEPENDENT-KINASES Articles GENOTOXIC STRESS Cell cycle Molecular biology Cyclin-Dependent Kinases CELL-CYCLE CONTROL 030104 developmental biology chemistry biology.protein RAD51 PARALOGS cell cycle IONIZING-RADIATION Homologous recombination MESSENGER-RNA DNA |
| Zdroj: | Weimer, A K, Biedermann, S, Harashima, H, Roodbarkelari, F, Takahashi, N, Foreman, J, Guan, Y, Pochon, G, Heese, M, Van Damme, D, Sugimoto, K, Koncz, C, Doerner, P, Umeda, M & Schnittger, A 2016, ' The plant specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis ', EMBO Journal, vol. 35, no. 19, pp. 2068-2086 . https://doi.org/10.15252/embj.201593083 EMBO JOURNAL Europe PubMed Central The EMBO Journal |
| ISSN: | 0261-4189 |
| DOI: | 10.15252/embj.201593083 |
| Popis: | Upon DNA damage, cyclin‐dependent kinases (CDKs) are typically inhibited to block cell division. In many organisms, however, it has been found that CDK activity is required for DNA repair, especially for homology‐dependent repair (HR), resulting in the conundrum how mitotic arrest and repair can be reconciled. Here, we show that Arabidopsis thaliana solves this dilemma by a division of labor strategy. We identify the plant‐specific B1‐type CDKs (CDKB1s) and the class of B1‐type cyclins (CYCB1s) as major regulators of HR in plants. We find that RADIATION SENSITIVE 51 (RAD51), a core mediator of HR, is a substrate of CDKB1‐CYCB1 complexes. Conversely, mutants in CDKB1 and CYCB1 fail to recruit RAD51 to damaged DNA. CYCB1;1 is specifically activated after DNA damage and we show that this activation is directly controlled by SUPPRESSOR OF GAMMA RESPONSE 1 (SOG1), a transcription factor that acts similarly to p53 in animals. Thus, while the major mitotic cell‐cycle activity is blocked after DNA damage, CDKB1‐CYCB1 complexes are specifically activated to mediate HR. |
| Databáze: | OpenAIRE |
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