IMPACT OF DONOR-SPECIFIC ANTIBODIES ON CHRONIC REJECTION OCCURRENCE AND GRAFT LOSS IN RENAL TRANSPLANTATION: POSTTRANSPLANT ANALYSIS USING FLOW CYTOMETRIC TECHNIQUES1
| Autor: | Simona Servetti, Nicola Torlone, Carlo Umberto Casciani, Domenico Adorno, Oreste Claudio Buonomo, Palmina I. Monaco, Elvira Poggi, Antonina Piazza, M. Valeri, L Borrelli |
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| Rok vydání: | 2001 |
| Předmět: |
Transplantation
medicine.medical_specialty Kidney Creatinine biology business.industry Human leukocyte antigen Gastroenterology Settore MED/18 - Chirurgia Generale chemistry.chemical_compound medicine.anatomical_structure Immune system Antigen chemistry Internal medicine Immunology medicine biology.protein Antibody Complication business |
| Zdroj: | Transplantation. 71:1106-1112 |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/00007890-200104270-00017 |
| Popis: | BACKGROUND Improvements in immunosuppressive therapy have greatly reduced acute rejection (ARj) episodes, ensuring better short-term graft outcome, but have not modified long-term survival in renal transplantation. It is now well accepted that chronic rejection (CRj) can be determined by both immune and/or nonimmune mechanisms. The aim of this study was to evaluate the importance of the posttransplant humoral immune response towards mismatched HLA graft antigens in CRj occurrence and graft outcome. METHODS Serum samples from 120 nonpresensitized renal transplant recipients were prospectively screened for 1 year after surgery by means of flow cytometry cross-match (FCXM) and FlowPRA beads (microbeads coated with purified HLA class I and class II antigens) assays. All transplants were followed-up for 2 years or until graft removal. RESULTS FCXM monitoring identified donor-specific antibodies (DS-Abs) in 29 (24.2%) of 120 transplanted patients. Correlation with clinical data highlighted a higher incidence of ARj in DS-Abs-positive patients compared to negative patients (62% vs. 13%, P |
| Databáze: | OpenAIRE |
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