Time Course of Degradation of Cardiac Troponin I in Patients With Acute ST-Elevation Myocardial Infarction
Autor: | Allan S. Jaffe, Jennifer E. Van Eyk, John H. Alexander, Lene Helleskov Madsen, Dan Atar, Geir Christensen, Christopher B. Granger, Ingvild B Hoen, Terje Lund, Zanina Grieg, Victor L. Serebruany |
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Rok vydání: | 2006 |
Předmět: |
Adult
Male medicine.medical_specialty Heart disease Physiology medicine.medical_treatment Blotting Western Myocardial Infarction Tenecteplase macromolecular substances Epitopes Fibrinolytic Agents Thrombolytic drug Internal medicine Troponin I medicine Humans Immunoprecipitation cardiovascular diseases Myocardial infarction Aged biology business.industry ST elevation Antibodies Monoclonal Thrombolysis Middle Aged musculoskeletal system medicine.disease Troponin Tissue Plasminogen Activator cardiovascular system biology.protein Cardiology Electrophoresis Polyacrylamide Gel Cardiology and Cardiovascular Medicine business medicine.drug |
Zdroj: | Circulation Research. 99:1141-1147 |
ISSN: | 1524-4571 0009-7330 |
DOI: | 10.1161/01.res.0000249531.23654.e1 |
Popis: | Although measurement of troponin is widely used for diagnosing acute myocardial infarction (AMI), its diagnostic potential may be increased by a more complete characterization of its molecular appearance and degradation in the blood. The aim of this study was to define the time course of cardiac troponin I (cTnI) degradation in patients with acute ST-elevation myocardial infarction (STEMI). In the ASSENT-2 substudy, 26 males hospitalized with STEMI were randomized to 2 different thrombolytic drugs within 6 hours after onset of symptoms. Blood samples were obtained just before initiation of thrombolysis and at 30 minutes intervals (7 samples per patient). Western blot analysis was performed using anti-cTnI antibodies and compared with serum concentrations of cTnI. All patients exceeded the cTnI cutoff for AMI during the sampling period; at initiation of therapy, 23 had elevated cTnI values. All patients demonstrated 2 bands on immunoblot: intact cTnI and a single degradation product as early as 90 minutes after onset of symptoms. On subsequent samples, 15 of 26 patients showed multiple degradation products with up to 7 degradation bands. The appearance of fragments was correlated with higher levels of cTnI ( P P =0.058). This study defines for the first time the initial time course of cTnI degradation in STEMI. Intact cTnI and a single degradation product were detectable on immunoblot as early as 90 minutes after onset of symptoms with further degradation after 165 minutes. Infarct size and time to initiation of treatment was the major determinant for degradation. |
Databáze: | OpenAIRE |
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