Serological evaluation of thin-layer immunoassay-enzyme-linked immunosorbent assay for antibody detection in human trichinellosis
| Autor: | Jorge-Luis de-la-Rosa, Lidia Crecencio-Rosales, Alberto Gómez-Priego |
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| Rok vydání: | 2000 |
| Předmět: |
Microbiology (medical)
food.ingredient Clinical Biochemistry Immunology Trichinella spiralis Antibodies Helminth Enzyme-Linked Immunosorbent Assay Trichinosis Sensitivity and Specificity Serology Antigen-Antibody Reactions Immunoenzyme Techniques food Antigen Double-Blind Method medicine Immunology and Allergy Agar Animals Humans medicine.diagnostic_test biology Trichinellosis biology.organism_classification medicine.disease Molecular biology Blot Immunoassay Antigens Helminth biology.protein Microbial Immunology Antibody |
| Zdroj: | Clinical and diagnostic laboratory immunology. 7(5) |
| ISSN: | 1071-412X |
| Popis: | A new immunoenzymatic test, named the thin-layer immunoassay–enzyme-linked immunosorbent assay (TIA-ELISA), was evaluated for antibody detection in human trichinellosis using excretion and secretion products prepared from Trichinella spiralis muscle larvae. Serum samples from people with positive muscle biopsies or symptoms compatible with the disease ( n = 8 or 26, respectively), all reactive in enzyme-linked immunoelectrotransfer blot assay (EITB), as well as 67 serum samples from healthy, EITB-negative people, were tested in an ELISA and TIA-ELISA. TIA-ELISA was performed in polystyrene plastic petri dishes by adding dots of 10 μl each of antigen (7 μg/ml) followed by adding diluted serum and the conjugate. Finally, the substrate mixed with agar was added to develop the reaction. Enzymatic by-products were easily detected by the naked eye as defined dots. Sensitivity and specificity were 76 and 94% for ELISA, and both parameters were 91% for TIA-ELISA. The kappa correlation indices for both tests in relation to EITB were 0.73 and 0.80, respectively. The TIA-ELISA can be carried out with common laboratory equipment in 3 h and uses lower quantities of antigen than EITB and ELISA. Since TIA-ELISA is easy to perform, cheap, sensitive, and specific, the test could be an acceptable alternative to use in clinical laboratories lacking specialized equipment needed for ELISA and EITB and in field studies for antibody detection in human trichinellosis. |
| Databáze: | OpenAIRE |
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