UPLC-ESI-MS/MS-based widely targeted metabolomics analysis of wood metabolites in teak (Tectona grandis)
| Autor: | Zaizhi Zhou, Kunnan Liang, Wang Xiyang, Yang Guang, Guihua Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
sapwood Pharmaceutical Science 01 natural sciences Analytical Chemistry Tectona grandis lcsh:QD241-441 03 medical and health sciences Metabolomics lcsh:Organic chemistry Drug Discovery Food science Physical and Theoretical Chemistry 030304 developmental biology 0303 health sciences biology Chemistry Organic Chemistry Uplc esi ms ms Primary metabolite Metabolite analysis biology.organism_classification Chemistry (miscellaneous) Tectona Molecular Medicine UPLC–ESI–MS/MS-based metabolomics heartwood 010606 plant biology & botany Targeted metabolomics |
| Zdroj: | Molecules, Vol 25, Iss 2189, p 2189 (2020) Molecules Volume 25 Issue 9 |
| ISSN: | 1420-3049 |
| Popis: | The properties of teak wood, such as natural durability and beautiful color, are closely associated with wood extractives. In order to further understand the performance differences between teak heartwood and sapwood, we analyzed the chemical components of extractives from 12 wood samples using an ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS)-based metabolomics approach. In total, 691 metabolites were identified, and these were classified into 17 different categories. Clustering analysis and principal component analysis of metabolites showed that heartwood samples could be clearly separated from sapwood samples. Differential metabolite analysis revealed that the levels of primary metabolites, including carbohydrates, amino acids, lipids, and nucleotides, were significantly lower in the heartwood than in the sapwood. Conversely, many secondary metabolites, including flavonoids, phenylpropanoids, and quinones, had higher levels in the heartwood than in the sapwood. In addition, we detected 16 specifically expressed secondary metabolites in the heartwood, the presence of which may correlate with the durability and color of teak heartwood. Our study improves the understanding of differential metabolites between sapwood and heartwood of teak, and provides a reference for the study of heartwood formation. |
| Databáze: | OpenAIRE |
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