An Overview of PulseNet USA Databases
| Autor: | Beth Tolar, Efrain M. Ribot, Kelley Hise, Steven Stroika, Morgan N Schroeder, Peter Gerner-Smidt, Lavin A. Joseph |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Databases Factual 040301 veterinary sciences Applied Microbiology and Biotechnology Microbiology Disease Outbreaks Foodborne Diseases 0403 veterinary science 03 medical and health sciences medicine Humans Public Health Surveillance Whole genome sequencing 0303 health sciences Whole Genome Sequencing 030306 microbiology Public health Review/Overview Articles Pulsenet 04 agricultural and veterinary sciences Data science United States Electrophoresis Gel Pulsed-Field Databases as Topic Animal Science and Zoology Public Health Business Laboratories Food Science |
| Zdroj: | Foodborne Pathogens and Disease. 16:457-462 |
| ISSN: | 1556-7125 1535-3141 |
| Popis: | PulseNet USA is the molecular surveillance network for foodborne disease in the United States. The network consists of state and local public health laboratories, as well as food regulatory agencies, that follow PulseNet's standardized protocols to perform pulsed-field gel electrophoresis (PFGE) and whole genome sequencing (WGS) and analyze the results using standardized software. The raw sequences are uploaded to the GenomeTrakr or PulseNet bioprojects at the National Center for Biotechnology Information. The PFGE patterns and analyzed sequence data are uploaded in real time with associated demographic data to the PulseNet national databases managed at the Centers for Disease Control and Prevention. The PulseNet databases are organism specific and provide a central storage location for molecular and demographic data related to an isolate. Sequences are compared in the databases, thereby facilitating the rapid detection of clusters of foodborne diseases that may represent widespread outbreaks. WGS genotyping data, for example, antibiotic resistance and virulence profiles, are also uploaded in real time to the PulseNet databases to improve food safety surveillance activities. |
| Databáze: | OpenAIRE |
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