Effects of Hexachlorocyclohexane (HCH-γ-Isomer, Lindane) Intoxication on the Proliferation and Apoptosis in Rat Testes
| Autor: | Hikmet Keleş, Erkan Karadaş, Hasan Hüseyin Demirel, Hayati Yüksel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
germ cells TUNEL assay lcsh:Veterinary medicine General Veterinary apoptosis Lindane Biology spermatogenesis chemistry.chemical_compound Endocrinology medicine.anatomical_structure Germ cell proliferation chemistry Apoptosis Internal medicine Toxicity medicine PCNA lcsh:SF600-1100 testosteron Spermatogenesis Germ cell Testosterone |
| Zdroj: | Acta Veterinaria Brno, Vol 78, Iss 4, Pp 615-620 (2009) |
| ISSN: | 1801-7576 0001-7213 |
| Popis: | In this study, experimentally lindane-induced histopathological changes and proliferation and/or apoptosis in germ cells in the rat testes were investigated. A total of 40 healthy fertile 3-month-old male rats were used. Animals were divided into 4 groups, each containing 10 rats. Group 1 (control) was given only pure olive oil, Groups 2, 3 and 4 were administered lindane at 10, 20 and 40 mg/kg/bw, respectively, by gastric gavage for 30 days. Microscopically, degenerative changes were observed in the lindane-treated groups. For proliferative activity PCNA immunolabelling and for germ cells apoptosis TUNEL methods were performed. Although a strong PCNA positivity in the control group was observed, a gradual decrease was noted in the lindane-treated groups especially at higher doses. Significant increases of apoptosis were seen in the lindane-treated groups compared to the control group. A decrease in testosterone concentrations was observed in lindane-treated groups compared to the control group. The study indicates that high-dose lindane intoxication contributes to the suppression of spermatogenesis through a reduction of germ cell proliferation and an increase of germ cell death in rat testes. |
| Databáze: | OpenAIRE |
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