Arguments against a close relationship between non-phosphorylating and phosphorylating glyceraldehyde-3-phosphate dehydrogenases
| Autor: | F.Della Seta, Guy Branlant, S. Michels, Sandra Scagliarini, Paolo Trost, M. Riva, C. Carles |
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| Rok vydání: | 1994 |
| Předmět: |
inorganic chemicals
Magnetic Resonance Spectroscopy Evolution Molecular Sequence Data Biophysics Aldehyde dehydrogenase Dehydrogenase Glyceraldehyde 3-Phosphate Biochemistry Cofactor Stereospecificity stomatognathic system Structural Biology Genetics Animals Amino Acid Sequence Phosphorylation Molecular Biology Peptide sequence Glyceraldehyde 3-phosphate dehydrogenase chemistry.chemical_classification Sequence Homology Amino Acid biology Molecular mass Glyceraldehyde-3-Phosphate Dehydrogenases Spinach Cell Biology Plants Biological Evolution NMR enzymes and coenzymes (carbohydrates) Enzyme chemistry biology.protein Glyceraldehyde-3-phosphate dehydrogenase NADP |
| Zdroj: | FEBS Letters. 339:97-100 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(94)80393-5 |
| Popis: | Non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.9) from spinach leaves was purified to homogeneity using an improved purification procedure. Thus, a major contaminant with molecular mass and ion-exchange properties similar to non-phosphorylating GAPDH was eliminated. Using this pure non-phosphorylating GAPDH, cofactor stereospecificity was determined by 1H NMR. Analysis of the NADPH formed from the hydride transfer from glyceraldehyde-3-phosphate to [4-2H]NADP showed that the enzyme belongs to the A-stereospecific dehydrogenase family. This stereospecificity is the same as that described for the aldehyde dehydrogenase (ALDH) superfamily and opposite to that of the phosphorylating GAPDH. Moreover, results from peptide sequencing analysis suggest a similarity in sequence between the non-phosphorylating GAPDH and ALDHs. Thus, the results taken all together strongly suggest that non-phosphorylating GAPDH belongs to the ALDH family and has no close relationship to the phosphorylating GAPDH class. |
| Databáze: | OpenAIRE |
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