VascuTrainer: A Mobile and Disposable Bioreactor System for the Conditioning of Tissue-Engineered Vascular Grafts
| Autor: | Diana M Rojas González, Ulrich Steinseifer, Christian Brecher, Petra Mela, Markus Obdenbusch, Stefan Jockenhoevel, Thomas Schmitz-Rode, Werner Herfs, Frederic Wolf |
|---|---|
| Přispěvatelé: | AMIBM, RS: FSE AMIBM, Sciences, RS: FSE Sciences, Biobased Materials, RS: FSE Biobased Materials |
| Rok vydání: | 2018 |
| Předmět: |
Tissue-engineered blood vessel
Endothelium Myocytes Smooth Muscle 0206 medical engineering Cell Culture Techniques Biomedical Engineering ISCHEMIA-REPERFUSION INJURY 02 engineering and technology ISCHEMIA/REPERFUSION INJURY Muscle Smooth Vascular Umbilical Arteries CULTURE Extracellular matrix Bioreactors Tissue engineering medicine Bioreactor Humans PROTECTION BLOOD-VESSEL Mechanical conditioning ARTERY Arterial substitute ENDOTHELIALIZATION Tissue Scaffolds Chemistry 021001 nanoscience & nanotechnology 020601 biomedical engineering Blood Vessel Prosthesis medicine.anatomical_structure Transportable bioreactor ENDOTHELIUM Fibrin scaffold CELLS Implant 0210 nano-technology PERFUSION BIOREACTOR Biomedical engineering Blood vessel Artery |
| Zdroj: | Annals of Biomedical Engineering, 46(4), 616-626. Springer, Cham |
| ISSN: | 1573-9686 0090-6964 |
| DOI: | 10.1007/s10439-018-1977-y |
| Popis: | In vitro tissue engineering of vascular grafts requires dynamic conditioning in a bioreactor system for in vitro tissue maturation and remodeling to receive a mechanically adequate and hemocompatible implant. The goal of the current work was to develop a bioreactor system for the conditioning of vascular grafts which is (i) able to create a wide range of flow, pressure and frequency conditions, including physiological ones; (ii) compact and easy to assemble; (iii) transportable; (iv) disposable. The system is driven by a small centrifugal pump controlled via a custom-made control unit, which can also be operated on batteries to allow for autonomous transportation. To show the potential of the newly developed bioreactor system small-caliber vascular composite grafts (n = 5, internal diameter = 3 mm, length = 12.5 cm) were fabricated using a fibrin scaffold embedding human umbilical artery smooth muscle cells and a polyvinylidene fluoride warp-knitted macroporous mesh. Subsequently, the vascular grafts were endothelialized and mounted in the bioreactor system for conditioning. The conditioning parameters remained within the predefined range over the complete conditioning period and during operation on batteries as tested for up to 25 h. Fabrication and pre-conditioning under arterial pressure and shear stress conditions resulted in robust and hemocompatible tissue-engineered vascular grafts. Analysis of immunohistochemical stainings against extracellular matrix and cell-specific proteins revealed collagen I and collagen III deposition. The luminal surface was confluently covered with endothelial cells. The developed bioreactor system showed cytocompatibility and pH, pO2, pCO2, glucose and lactate stayed constant. Sterility was maintained during the complete fabrication process of the vascular grafts. The potential of a versatile and mobile system and its functionality by conditioning tissue-engineered vascular grafts under physiological pressure and flow conditions could be demonstrated. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink