A conformational change of C-reactive protein in burn wounds unmasks its proinflammatory properties
| Autor: | Steffen U. Eisenhardt, Hans-Georg Koch, Jan R. Thiele, Karlheinz Peter, David Braig, Holger Bannasch, G. Bjoern Stark, Benedict Kaiser |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Keratinocytes
Necrosis Protein Conformation Immunology Apoptosis Inflammation Monocytes Cell Line Proinflammatory cytokine Phagocytosis Western blot Cell Movement medicine Humans Immunology and Allergy Platelet activation Skin Wound Healing biology medicine.diagnostic_test Macrophages C-reactive protein Acute-phase protein General Medicine Molecular biology C-Reactive Protein biology.protein medicine.symptom Burns Reactive Oxygen Species Wound healing |
| Zdroj: | International Immunology. 26:467-478 |
| ISSN: | 1460-2377 0953-8178 |
| DOI: | 10.1093/intimm/dxu056 |
| Popis: | Tissue damage in burn injury leads to a rapid increase of leukocytes and acute phase reactants. Plasma levels of C-reactive protein (CRP) rise within hours after the insult. No deficiency of this protein has been reported in humans, suggesting it plays a pivotal role in innate immunity. CRP in circulation is composed of five identical subunits [pentameric CRP (pCRP)]. Recently, deposits of structurally modified CRP (mCRP) have been found in inflammatory diseases. Little is known about this structural change and how it affects CRP functions. We analyzed CRP deposits in burn wounds and serum by immunohistochemistry, western blot and dot blot analysis. CRP was deposited in necrotic and inflamed tissue, but not in adjacent healthy tissue. Tissue deposited CRP was detected by mCRP-specific antibodies and structurally different from serum pCRP. mCRP but not pCRP induced reactive oxygen species production by monocytes and facilitated uptake of necrotic Jurkat cells by macrophages. In addition, it accelerated migration of keratinocytes in a scratch wound assay. The structural changes that occur in pCRP upon localization to damaged and inflamed tissue in burn wounds result in a functionally altered protein with distinct functions. mCRP exhibits opsonic, proinflammatory and promigratory properties which modulate wound healing. |
| Databáze: | OpenAIRE |
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