Fermentation of rice bran hydrolysate to ethanol using Zymomonas mobilis biofilm immobilization on DEAE-cellulose
| Autor: | Pornthap Thanonkeo, Tatsaporn Todhanakasem, Kamonchanok Areerat, Tipong Narkmit |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Ethanol
Bran biology lcsh:Biotechnology Biofilm Zymomonas mobilis biochemical phenomena metabolism and nutrition biology.organism_classification Applied Microbiology and Biotechnology Hydrolysate chemistry.chemical_compound chemistry Biochemistry lcsh:Biology (General) Ethanol production Yield (chemistry) lcsh:TP248.13-248.65 Rice bran Ethanol fuel Fermentation Food science lcsh:QH301-705.5 Biotechnology |
| Zdroj: | Electronic Journal of Biotechnology, Volume: 18, Issue: 3, Pages: 196-201, Published: MAY 2015 Electronic Journal of Biotechnology, Vol 18, Iss 3, Pp 196-201 (2015) Electronic Journal of Biotechnology v.18 n.3 2015 SciELO Chile CONICYT Chile instacron:CONICYT |
| Popis: | Background: Major challenges associated with the fermentation of lignocellulosic hydrolysates are the reduction in the operating cost and minimizing the complexity of the process. Zymomonas mobilis biofilm has been emerged to resolve these complexities. Biofilm has been reported to tolerate to the toxic inhibitors and easily manipulated toward the cell recycle through the cell immobilization. Results: Z. mobilis ZM4 and TISTR 551 were able to develop biofilms on DEAE cellulose under the differences in the morphologies. Z. mobilis ZM4 developed homogeneous biofilm that brought DEAE fiber to be crosslinking, while Z .mobilis TISTR 551 developed heterogeneous biofilm in which crosslinking was not observed. Ethanol production under batch and repeated batch fermentation of rice bran hydrolysate containing toxic inhibitors were compared between these two biofilms. TISTR 551 biofilm produced the maximum yield (Y P/S ) of 0.43 ± 0.09 g ethanol/ g glucose (83.89% theoretical yield). However the repeated batch could not be proceeded due to the bacterial detachment. Z. mobilis ZM4 biofilm produced the maximum yield (Y P/S ) of 0.177 ± 0.05 g ethanol/ g glucose (34.74% theoretical yield) in the batch culture and the biofilm remained intact to proceed along the repeated batch. The highest ethanol yield (Y P/S ) in the repeated batch of Z. mobilis ZM4 was 0.354 ± 0.07 g ethanol/ g glucose (69.51% theoretical yield). Conclusions: Homogeneous biofilm structure of Z. mobilis provided more recycle beneficial over the heterogeneous biofilm structure for the ethanol production from lignocellulosic hydrolysate. Normal 0 21 false false false EN-US X-NONE TH st1\:*{behavior:url(#ieooui) } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} |
| Databáze: | OpenAIRE |
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