Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle
| Autor: | Leonardo Mariño-Ramírez, Ellie Anne López, Pilar Rodríguez-Becerra, Andrés Pinzón-Velasco, Javier Hernández-Fernández |
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| Rok vydání: | 2021 |
| Předmět: |
Health
Toxicology and Mutagenesis 010501 environmental sciences Biology lcsh:Chemical technology Toxicology medicine.disease_cause 01 natural sciences Article differential expression Transcriptome Quelónidos transcriptomics 03 medical and health sciences Differential expression Downregulation and upregulation Gene expression medicine lcsh:TP1-1185 Gene 030304 developmental biology 0105 earth and related environmental sciences chemistry.chemical_classification Caretta caretta 0303 health sciences Reactive oxygen species Chemical Health and Safety Tortugas Ácido ribonucleico -- Síntesis Autophagy methylmercury Cell biology chemistry Apoptosis RNA-seq Oxidative stress |
| Zdroj: | Toxics Volume 9 Issue 4 Expeditio: repositorio UTadeo Universidad de Bogotá Jorge Tadeo Lozano instacron:Universidad de Bogotá Jorge Tadeo Lozano Toxics, Vol 9, Iss 70, p 70 (2021) |
| ISSN: | 2305-6304 |
| DOI: | 10.3390/toxics9040070 |
| Popis: | To understand changes in enzyme activity and gene expression as biomarkers of exposure to methylmercury, we exposed loggerhead turtle erythrocytes (RBCs) to concentrations of 0, 1, and 5 mg L−1 of MeHg and de novo transcriptome were assembled using RNA-seq. The analysis of differentially expressed genes (DEGs) indicated that 79 unique genes were dysregulated (39 upregulated and 44 downregulated genes). The results showed that MeHg altered gene expression patterns as a response to the cellular stress produced, reflected in cell cycle regulation, lysosomal activity, autophagy, calcium regulation, mitochondrial regulation, apoptosis, and regulation of transcription and translation. The analysis of DEGs showed a low response of the antioxidant machinery to MeHg, evidenced by the fact that genes of early response to oxidative stress were not dysregulated. The RBCs maintained a constitutive expression of proteins that represented a good part of the defense against reactive oxygen species (ROS) induced by MeHg. |
| Databáze: | OpenAIRE |
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