Effect of Early Peptide Diets on Zebrafish Skeletal Development

Autor: Alice Printzi, George Koumoundouros, Vincent Fournier, Lauriane Madec, Jose-Luis Zambonino-Infante, David Mazurais
Přispěvatelé: Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), University of Crete [Heraklion] (UOC), DIANA AQUA
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Biomolecules (2218-273X) (MDPI AG), 2023-04, Vol. 13, N. 4, P. 659 (15p.)
Biomolecules
Biomolecules, 2023, 13 (4), pp.659. ⟨10.3390/biom13040659⟩
Biomolecules; Volume 13; Issue 4; Pages: 659
ISSN: 2218-273X
DOI: 10.3390/biom13040659⟩
Popis: International audience; Incorporation of dietary peptides has been correlated with decreased presence of skeletal abnormalities in marine larvae. In an attempt to clarify the effect of smaller protein fractions on fish larval and post-larval skeleton, we designed three isoenergetic diets with partial substitution of their protein content with 0% (C), 6% (P6) and 12% (P12) shrimp di-and tripeptides. Experimental diets were tested in zebrafish under two regimes, with inclusion (ADF-Artemia and dry feed) or lack (DF-dry feed only) of live food. Results at the end of metamorphosis highlight the beneficial effect of P12 on growth, survival and early skeletal quality when dry diets are provided from first feeding (DF). Exclusive feeding with P12 also increased the musculoskeletal resistance of the postlarval skeleton against the swimming challenge test (SCT). On the contrary, Artemia inclusion (ADF) overruled any peptide effect in total fish performance. Given the unknown species' larval nutrient requirements, a 12% dietary peptide incorporation is proposed for successful rearing without live food. A potential nutritional control of the larval and post-larval skeletal development even in aquaculture species is suggested. Limitations of the current molecular analysis are discussed to enable the future identification of the peptide-driven regulatory pathways.
Databáze: OpenAIRE
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